Applications of western blot technique: From bench to bedside

被引:46
作者
Meftahi, Gholam Hossein [1 ]
Bahari, Zahra [2 ]
Mahmoudabadi, Ali Zarei [3 ]
Iman, Maryam [4 ]
Jangravi, Zohreh [3 ,5 ]
机构
[1] Baqiyatallah Univ Med Sci, Neurosci Res Ctr, Tehran, Iran
[2] Baqiyatallah Univ Med Sci, Fac Med, Dept Physiol & Med Phys, Tehran, Iran
[3] Baqiyatallah Univ Med Sci, Fac Med, Dept Biochem, POB 19395-5485, Tehran, Iran
[4] Baqiyatallah Univ Med Sci, Fac Pharm, Dept Pharmaceut, Tehran, Iran
[5] Baqiyatallah Univ Med Sci, Nanobiotechnol Res Ctr, Tehran, Iran
关键词
antibody; clinical application; protein immunoblot; scientific application; western blot; PROTEIN-PROTEIN INTERACTIONS; MONOCLONAL-ANTIBODIES; DEMETHYLASE; 5D; DIAGNOSIS; ANTIGENS; IGG; IDENTIFICATION; TUBERCULOSIS; PERFORMANCE; BIOMARKERS;
D O I
10.1002/bmb.21516
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Western blot (WB) or immunoblot is a workhorse method. It is commonly used by biologists for study of different aspects of protein biomolecules. In addition, it has been widely used in disease diagnosis. Despite some limitations such as long time, different applications of WB have not been limited. In the present review, we have summarized scientific and clinical applications of WB. In addition, we described some new generation of WB techniques.
引用
收藏
页码:509 / 517
页数:9
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