A Novel Mechanism of S-equol Action in Neurons and Astrocytes: The Possible Involvement of GPR30/GPER1

被引:25
作者
Ariyani, Winda [1 ]
Miyazaki, Wataru [1 ,2 ]
Koibuchi, Noriyuki [1 ]
机构
[1] Gunma Univ, Grad Sch Med, Dept Integrat Physiol, 3-39-22 Showa Machi Maebashi, Gunma 3718511, Japan
[2] Hirosaki Univ, Grad Sch Hlth Sci, Dept Biosci & Lab Med, 66-1 Hon Cho, Hirosaki, Aomori 0368564, Japan
关键词
soy isoflavone; S-equol; estrogen receptor; GPR30; neuron; astrocyte; COUPLED ESTROGEN-RECEPTOR; THYROID-HORMONE; MENOPAUSAL SYMPTOMS; GENE-EXPRESSION; GPR30; GROWTH; G-PROTEIN-COUPLED-RECEPTOR-30; JAPANESE; BRAIN; SUPPLEMENT;
D O I
10.3390/ijms20205178
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
S-equol is a major bacterial metabolite of the soy isoflavone daidzein. It is known to be a phytoestrogen that acts by binding to the nuclear estrogen receptors (ERs) that are expressed in various brain regions, including the cerebellum. However, the effects of S-equol on cerebellar development and function have not yet been extensively studied. In this study, the effects of S-equol were evaluated using a mouse primary cerebellar culture, Neuro-2A clonal cells, and an astrocyte-enriched culture. S-equol augmented the dendrite arborization of Purkinje cells induced by triiodothyronine (T-3) and the neurite growth of Neuro-2A cell differentiation. Such augmentation was suppressed by G15, a selective G-protein coupled ER (GPR30) antagonist, and ICI 182,780, an antagonist for ERs in both cultures. On the other hand, in astrocytes, S-equol induced cell proliferation and cell migration with an increase in the phosphorylated extracellular-signal-regulated kinase 1/2 and F-actin rearrangements. Such effects were suppressed by G15, but not by ICI. These findings indicated that S-equol may enhanced cerebellar development by affecting both neurons and astrocytes through several signaling pathways, including GPR30 and ERs. We here report a novel mechanism of S-equol in cerebellar development that may provide a novel possibility to use S-equol supplementation during development.
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页数:14
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