Novel potential serological prostate cancer biomarkers using CT100+cancer antigen microarray platform in a multi-cultural South African cohort

被引:32
作者
Adeola, Henry A. [1 ,2 ]
Smith, Muneerah [2 ]
Kaestner, Lisa [3 ]
Blackburn, Jonathan M. [2 ]
Zerbini, Luiz F. [1 ,2 ]
机构
[1] Int Ctr Genet Engn & Biotechnol, Cape Town, South Africa
[2] Univ Cape Town, Fac Hlth Sci, Div Med Biochem, Inst Infect Dis & Mol Med, ZA-7925 Cape Town, South Africa
[3] Grootes Schuur Hosp, Dept Urol, Cape Town, South Africa
基金
新加坡国家研究基金会;
关键词
serum; prostate cancer; biomarkers; protein microarray; cancer-testis antigen; TUMOR-ASSOCIATED ANTIGENS; RENAL LYMPH-NODE; CANCER/TESTIS ANTIGENS; PROTEIN MICROARRAYS; ACID-PHOSPHATASE; MINI-ARRAY; EXPRESSION; URINE; SERUM; RISK;
D O I
10.18632/oncotarget.7359
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
There is a growing need for high throughput diagnostic tools for early diagnosis and treatment monitoring of prostate cancer (PCa) in Africa. The role of cancer-testis antigens (CTAs) in PCa in men of African descent is poorly researched. Hence, we aimed to elucidate the role of 123 Tumour Associated Antigens (TAAs) using antigen microarray platform in blood samples (N = 67) from a South African PCa, Benign prostatic hyperplasia (BPH) and disease control (DC) cohort. Linear (fold-over-cutoff) and differential expression quantitation of autoantibody signal intensities were performed. Molecular signatures of candidate PCa antigen biomarkers were identified and analyzed for ethnic group variation. Potential cancer diagnostic and immunotherapeutic inferences were drawn. We identified a total of 41 potential diagnostic/therapeutic antigen biomarkers for PCa. By linear quantitation, four antigens, GAGE1, ROPN1, SPANXA1 and PRKCZ were found to have higher autoantibody titres in PCa serum as compared with BPH where MAGEB1 and PRKCZ were highly expressed. Also, p53 S15A and p53 S46A were found highly expressed in the disease control group. Statistical analysis by differential expression revealed twenty-four antigens as upregulated in PCa samples, while 11 were downregulated in comparison to BPH and DC (FDR = 0.01). FGFR2, COL6A1 and CALM1 were verifiable biomarkers of PCa analysis using urinary shotgun proteomics. Functional pathway annotation of identified biomarkers revealed similar enrichment both at genomic and proteomic level and ethnic variations were observed. Cancer antigen arrays are emerging useful in potential diagnostic and immunotherapeutic antigen biomarker discovery.
引用
收藏
页码:13945 / 13964
页数:20
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