Biowire platform for maturation of human pluripotent stem cell-derived cardiomyocytes

被引：57

作者：

Sun, Xuetao ^{[1
]}

Nunes, Sara S. ^{[1
,2
,3
]}

机构：

[1] Univ Hlth Network, Toronto Gen Res Inst, 101 Coll St,MaRS,TMDT 3-904, Toronto, ON M5G 1L7, Canada

[2] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 1A1, Canada

[3] Univ Toronto, Heart & Stroke Richard Lewar Ctr Excellence, Toronto, ON M5S 1A1, Canada

来源：

METHODS | 2016年 / 101卷

基金：

加拿大健康研究院;

关键词：

Stem cells; Cardiomyocyte; Tissue engineering; Maturation; Biomimetic cues; DIFFERENTIATION; MYOCARDIUM;

D O I：

10.1016/j.ymeth.2015.11.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human pluripotent stem cells (hPSCs)-derived cardiomyocytes (hPSC-CMs) represent a potential indefinite cell supply for cardiac tissue engineering and possibly regenerative medicine applications. However, these cells are immature compared with adult ventricular cardiomyocytes. In order to overcome this limitation, an engineered platform, called biowire, was devised to provide cultured cardiomyocytes important biomimetic cues present during embryo development, such as three-dimensional cell culture, extracellular matrix composition, soluble factors and pacing through electrical stimulation, to induce the maturation of hPSC-CMs in vitro. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：21 / 26

页数：6

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