Hydroxychloroquine-conjugated gold nanoparticles for improved siRNA activity

被引：46
作者：
Perche, F. [1 ,2 ,3 ]
Yi, Y. [1 ]
Hespel, L. [1 ]
Mi, P. [1 ]
Dirisala, A. [1 ]
Cabral, H. [1 ]
Miyata, K. [1 ]
Kataoka, K. [1 ]
机构：
[1] Univ Tokyo, Grad Sch Engn, Dept Mat Engn, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138856, Japan
[2] CNRS, UPR4301, INSERM, Ctr Biophys Mol, F-45071 Orleans 02, France
[3] Univ Orleans, F-45071 Orleans 02, France
来源：
BIOMATERIALS | 2016年 / 90卷
关键词：
siRNA delivery; Hydroxychloroquine; Gold nanoparticles; Intracellular bioavailability; SMALL INTERFERING RNA; INTRACELLULAR DELIVERY; POLYMERIC MICELLES; DRUG-DELIVERY; GENE-TRANSFER; IDENTIFICATION; LOCALIZATION; AGGREGATION; TRAFFICKING; MECHANISM;
D O I：
10.1016/j.biomaterials.2016.02.027
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Current technology of siRNA delivery relies on pharmaceutical dosage forms to route maximal doses of siRNA to the tumor. However, this rationale does not address intracellular bottlenecks governing silencing activity. Here, we tested the impact of hydroxychloroquine conjugation on the intracellular fate and silencing activity of siRNA conjugated PEGylated gold nanoparticles. Addition of hydroxychloroquine improved endosomal escape and increased siRNA guide strand distribution to the RNA induced silencing complex (RISC), both crucial obstacles to the potency of siRNA. This modification significantly improved gene downregulation in cellulo. Altogether, our data suggest the benefit of this modification for the design of improved siRNA delivery systems. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：62 / 71
页数：10
相关论文
共 56 条
  • [1] Enhanced Gene and siRNA Delivery by Polycation-Modified Mesoporous Silica Nanoparticles Loaded with Chloroquine
    Bhattarai, Shanta Raj
    Muthuswamy, Elayaraja
    Wani, Amit
    Brichacek, Michal
    Castaneda, Antonio L.
    Brock, Stephanie L.
    Oupicky, David
    [J]. PHARMACEUTICAL RESEARCH, 2010, 27 (12) : 2556 - 2568
  • [2] Quantitative Evaluation of Cellular Uptake and Trafficking of Plain and Polyethylene Glycol-Coated Gold Nanoparticles
    Brandenberger, Christina
    Muehlfeld, Christian
    Ali, Zulqurnain
    Lenz, Anke-Gabriele
    Schmid, Otmar
    Parak, Wolfgang J.
    Gehr, Peter
    Rothen-Rutishauser, Barbara
    [J]. SMALL, 2010, 6 (15) : 1669 - 1678
  • [3] Cabral H, 2011, NAT NANOTECHNOL, V6, P815, DOI [10.1038/nnano.2011.166, 10.1038/NNANO.2011.166]
  • [4] Systemic Targeting of Lymph Node Metastasis through the Blood Vascular System by Using Size-Controlled Nano carriers
    Cabral, Horacio
    Makino, Jun
    Matsumoto, Yu
    Mi, Peng
    Wu, Hailiang
    Nomoto, Takahiro
    Toh, Kazuko
    Yamada, Naoki
    Higuchi, Yuriko
    Konishi, Satoshi
    Kano, Mitsunobu R.
    Nishihara, Hiroshi
    Miura, Yutaka
    Nishiyama, Nobuhiro
    Kataoka, Kazunori
    [J]. ACS NANO, 2015, 9 (05) : 4957 - 4967
  • [5] Real-time quantification of microRNAs by stem-loop RT-PCR
    Chen, CF
    Ridzon, DA
    Broomer, AJ
    Zhou, ZH
    Lee, DH
    Nguyen, JT
    Barbisin, M
    Xu, NL
    Mahuvakar, VR
    Andersen, MR
    Lao, KQ
    Livak, KJ
    Guegler, KJ
    [J]. NUCLEIC ACIDS RESEARCH, 2005, 33 (20) : e179.1 - e179.9
  • [6] Mechanism for the endocytosis of spherical nucleic acid nanoparticle conjugates
    Choi, Chung Hang J.
    Hao, Liangliang
    Narayan, Suguna P.
    Auyeung, Evelyn
    Mirkin, Chad A.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (19) : 7625 - 7630
  • [7] Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles
    Coulter, Jonathan A.
    Jain, Suneil
    Butterworth, Karl T.
    Taggart, Laura E.
    Dickson, Glenn R.
    McMahon, Stephen J.
    Hyland, Wendy B.
    Muir, Mark F.
    Trainor, Coleman
    Hounsell, Alan R.
    O'Sullivan, Joe M.
    Schettino, Giuseppe
    Currell, Fred J.
    Hirst, David G.
    Prise, Kevin M.
    [J]. INTERNATIONAL JOURNAL OF NANOMEDICINE, 2012, 7 : 2673 - 2685
  • [8] Gold Nanoparticles for Nucleic Acid Delivery
    Ding, Ya
    Jiang, Ziwen
    Saha, Krishnendu
    Kim, Chang Soo
    Kim, Sung Tae
    Landis, Ryan F.
    Rotello, Vincent M.
    [J]. MOLECULAR THERAPY, 2014, 22 (06) : 1075 - 1083
  • [9] Aggregation and Precipitation of Gold Nanoparticle Clusters in Carbon Dioxide-Gas-Expanded Liquid Dimethyl Sulfoxide
    Duggan, Jennifer N.
    Roberts, Christopher B.
    [J]. JOURNAL OF PHYSICAL CHEMISTRY C, 2014, 118 (26) : 14595 - 14605
  • [10] Nanomedicine(s) under the Microscope
    Duncan, Ruth
    Gaspar, Rogerio
    [J]. MOLECULAR PHARMACEUTICS, 2011, 8 (06) : 2101 - 2141
123456