Simultaneous determination of omeprazole and domperidone in capsules by RP-HPLC and densitometric HPTLC

A rapid, simple, and sensitive HPLC and a densitometric HPTLC method for the determination of omeprazole and domperidone in capsule formulations were developed and validated. For HPLC, the separation of components was achieved on a Phenomenex Rp-C-18 column. Isocratic elution with a mobile phase consisting of 0.01 M pH 6.5, ammonium acetate buffer: methanol: acetonitrile (40:30:30 v/v, pH 7.44 +/- 0.02), at a flow rate 1.0 mL/min was employed. Rabeprazole was used as the internal standard. In densitometric HPTLC, separation was achieved on aluminum sheets of silica gel 60F(254) using ethyl acetate: methanol: benzene (40:20:40 v/v) as mobile phase. Linear concentration range of HPLC and HPTLC methods were between 400-2000 ng/mL and 120-360 ng/spot for both the drugs, respectively. In HPLC, the detection limit was 131.27 ng/mL for omeprazole and 131.20 ng/mL for domperidone, the mean analytical recovery in determination of omeprazole and domperidone capsules was 99.14 +/- 1.81 for omeprazole and 99.63 +/- 1.68 for domperidone. Whereas, the detection limit was 40.83 ng/spot for orneprazole and 40.53 ng/spot for domperidone, the mean analytical recovery in determination of orneprazole and domperidone capsules was 99.51 +/- 0.91 and 99.48 +/- 1.15, respectively, in HPTLC. The components were detected by UV detection at 295 nm. Thus, the proposed method is applicable for routine determination of omeprazole and domperidone in pharmaceutical formulations.