Heparan sulfate proteoglycan expression in the regenerating zebrafish fin

被引:13
作者
Keil, Sebastian [1 ,2 ]
Gupta, Mansi [1 ,3 ]
Brand, Michael [1 ]
Knopf, Franziska [1 ,2 ]
机构
[1] Tech Univ Dresden, CRTD Ctr Regenerat Therapies TU Dresden, Dresden, Germany
[2] Tech Univ Dresden, Ctr Hlth Aging TU Dresden, Dresden, Germany
[3] Merus NV, Utrecht, Netherlands
基金
欧盟地平线“2020”;
关键词
extracellular matrix protein; glypican; growth factor; syndecan;
D O I
10.1002/dvdy.321
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Background Heparan sulfate proteoglycan (HSPG) expression is found in many animal tissues and regulates growth factor signaling such as of Fibroblast growth factors (Fgf), Wingless/Int (Wnt) and Hedgehog (HH). Glypicans, which are GPI (glycosylphosphatidylinositol)-anchored proteins, and transmembrane-anchored syndecans represent two major HSPG protein families whose involvement in development and disease has been demonstrated. Their participation in regenerative processes both of the central nervous system and of regenerating limbs is well documented. However, whether HSPG are expressed in regenerating zebrafish fins, is currently unknown. Results Here, we carried out a systematic screen of glypican and syndecan mRNA expression in regenerating zebrafish fins during the outgrowth phase. We find that 8 of the 10 zebrafish glypicans and the three known zebrafish syndecans show specific expression at 3 days post amputation. Expression is found in different domains of the regenerate, including the distal and lateral basal layers of the wound epidermis, the distal most blastema and more proximal blastema regions. Conclusions HSPG expression is prevalent in regenerating zebrafish fins. Further research is needed to delineate the function of glypican and syndecan action during zebrafish fin regeneration.
引用
收藏
页码:1368 / 1380
页数:13
相关论文
共 80 条
[21]   Syndecans-1 and -4 are induced during wound repair of neonatal but not fetal skin [J].
Gallo, R ;
Kim, C ;
Kokenyesi, R ;
Adzick, NS ;
Bernfield, M .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1996, 107 (05) :676-683
[22]   Regulation of Regeneration by Heparan Sulfate Proteoglycans in the Extracellular Matrix [J].
Gardiner D.M. .
Regenerative Engineering and Translational Medicine, 2017, 3 (3) :192-198
[23]   Syndecans in Inflammation at a Glance [J].
Gopal, Sandeep .
FRONTIERS IN IMMUNOLOGY, 2020, 11
[24]   Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration [J].
Govindan, Jayalakshmi ;
Iovine, M. Kathryn .
GENE EXPRESSION PATTERNS, 2015, 19 (1-2) :21-29
[25]   Glypican-3 modulates BMP- and FGP-mediated effects during renal branching morphogenesis [J].
Grisaru, S ;
Cano-Gauci, D ;
Tee, J ;
Filmus, J ;
Rosenblum, ND .
DEVELOPMENTAL BIOLOGY, 2001, 231 (01) :31-46
[26]   Notch signaling coordinates cellular proliferation with differentiation during zebrafish fin regeneration [J].
Grotek, Bartholomaeus ;
Wehner, Daniel ;
Weidinger, Gilbert .
DEVELOPMENT, 2013, 140 (07) :1412-1423
[27]   Identification and Expression Analysis of Zebrafish Glypicans during Embryonic Development [J].
Gupta, Mansi ;
Brand, Michael .
PLOS ONE, 2013, 8 (11)
[28]  
Gurley K.A., 2008, StemBook
[29]   Heparan sulphate proteoglycans:: The sweet side of development [J].
Häcker, U ;
Nybakken, K ;
Perrimon, N .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2005, 6 (07) :530-541
[30]   Distinct expression patterns of syndecans in the embryonic zebrafish brain [J].
Hofmeister, Wolfgang ;
Devine, Christine A. ;
Key, Brian .
GENE EXPRESSION PATTERNS, 2013, 13 (3-4) :126-132