Global profiling of distinct cysteine redox forms reveals wide-ranging redox regulation in C. elegans

被引:69
作者
Meng, Jin [1 ,2 ,3 ]
Fu, Ling [4 ,5 ]
Liu, Keke [4 ]
Tian, Caiping [4 ,6 ]
Wu, Ziyun [1 ,2 ,3 ]
Jung, Youngeun [7 ]
Ferreira, Renan B. [7 ]
Carroll, Kate S. [7 ]
Blackwell, T. Keith [1 ,2 ,3 ]
Yang, Jing [4 ,5 ]
机构
[1] Joslin Diabet Ctr, Div Res, 1 Joslin Pl, Boston, MA 02215 USA
[2] Harvard Med Sch, Dept Genet, Boston, MA 02115 USA
[3] Harvard Stem Cell Inst, Cambridge, MA 02138 USA
[4] Natl Ctr Prot Sci Beijing, Beijing Proteome Res Ctr, Beijing Inst Life, State Key Lab Prote, Beijing, Peoples R China
[5] Qingdao Univ, Med Coll, Med Sch, Innovat Inst, Qingdao, Peoples R China
[6] Tsinghua Univ, Sch Med, Beijing, Peoples R China
[7] Scripps Res Inst, Dept Chem, Jupiter, FL USA
基金
国家重点研发计划;
关键词
D O I
10.1038/s41467-021-21686-3
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Post-translational changes in the redox state of cysteine residues can rapidly and reversibly alter protein functions, thereby modulating biological processes. The nematode C. elegans is an ideal model organism for studying cysteine-mediated redox signaling at a network level. Here we present a comprehensive, quantitative, and site-specific profile of the intrinsic reactivity of the cysteinome in wild-type C. elegans. We also describe a global characterization of the C. elegans redoxome in which we measured changes in three major cysteine redox forms after H2O2 treatment. Our data revealed redox-sensitive events in translation, growth signaling, and stress response pathways, and identified redox-regulated cysteines that are important for signaling through the p38 MAP kinase (MAPK) pathway. Our in-depth proteomic dataset provides a molecular basis for understanding redox signaling in vivo, and will serve as a valuable and rich resource for the field of redox biology. Reversible cysteine oxidative modifications have emerged as important mechanisms that alter protein function. Here the authors globally assess the cysteine reactivity and an array of cysteine oxidative modifications in C. elegans, providing insights into redox signaling at the organismal level.
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页数:13
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