Patterns of thyroid hormone receptor expression in zebrafish and generation of a novel model of resistance to thyroid hormone action

Resistance to thyroid hormone can be due to heterozygous, dominant negative (DN) THRA (RTH alpha) or THRB (RTH beta) mutations, but the underlying mechanisms are incompletely understood. Here, we delineate the spatiotemporal expression of TH receptors (TRs) in zebrafish and generated morphants expressing equivalent amounts of wild-type and DN TR alpha s (thraa_MOs) and TR beta s (thrb_MOs) in vivo. Both morphants show severe developmental abnormalities. The phenotype of thraa_MOs includes brain and cardiac defects, but normal thyroid volume and tshba expression. A combined modification of dio2 and dio3 expression can explain the high T3/T4 ratio seen in thraa_MOs, as in RTH alpha. Thrb_MOs show abnormal eyes and otoliths, with a typical RTH beta pattern of thyroid axis. The coexpression of wild-type, but not mutant, human TRs can rescue the phenotype in both morphants. High T3 doses can partially revert the dominant negative action of mutant TRs in morphant fish. Therefore, our morphants recapitulate the RTH alpha and RTH beta key manifestations-representing new models in which the functional consequences of human TR mutations can be rapidly and faithfully evaluated. (C) 2016 Elsevier Ireland Ltd. All rights reserved.