Effect of bone marrow derived mesenchymal stem cells on lung pathology and inflammation in ovalbumin-induced asthma in mouse

被引:3
作者
Mohammadian, Maryam [1 ]
Boskabady, Mohammad Hosein [2 ,3 ]
Kashani, Iraj Ragerdi [4 ]
Jahromi, Gila Pirzad [5 ]
Omidi, Amene [4 ]
Nejad, Amir Kavian [6 ]
Khamse, Safoura [1 ]
Sadeghipour, Hamid Reza [1 ]
机构
[1] Univ Tehran Med Sci, Sch Med, Dept Physiol, Tehran, Iran
[2] Mashhad Univ Med Sci, Neurogen Inflammat Res Ctr, Mashhad, Iran
[3] Mashhad Univ Med Sci, Sch Med, Dept Physiol, Mashhad, Iran
[4] Univ Tehran Med Sci, Sch Med, Dept Anat, Tehran, Iran
[5] Baqiyatallah Univ Med Sci, Neurosci Res Ctr, Tehran, Iran
[6] Shahid Beheshti Univ Med Sci, Sch Nursing & Midwifery, Dept Emergency Med Serv, Tehran, Iran
关键词
Inflammation; Lung pathology; Ovalbumin-; induced; asthma; Stem cells; ALLERGIC AIRWAY INFLAMMATION; STROMAL CELLS; MURINE MODEL; GUINEA-PIGS; SUPPRESSION; FIBROSIS; TISSUE; IMMUNOMODULATION; MODULATION; MIGRATION;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Objective(s): Bone marrow-derived mesenchymal stem cells (BMSCs) have attracted significant interest to treat asthma and its complication. In this study, the effects of BMSCs on lung pathology and Inflammation in an ovalbumin-induced asthma model in mouse were examined. Materials and Methods: BALB/c mice were divided into three groups: control group (animals were not sensitized), asthma group (animals were sensitized by ovalbumin), asthma+BMSC group (animals were sensitized by ovalbumin and treated with BMSCs). BMSCs were isolated and characterized and then labeled with Bromodeoxyuridine (BrdU). After that the cells transferred into asthmatic mice. Histopathological changes of the airways, BMSCs migration and total and differential white blood cell (WBC) count in bronchoalveolar lavage (BAL) fluid were evaluated. Results: A large number of BrdU-BMSCs were found in the lungs of mice treated with BMSCs. The histopathological changes, BAL total WBC counts and the percentage of neutrophils and eosinophils were increased in asthma group compared to the control group. Treatment with BMSCs significantly decreased airway pathological indices, inflammatory cell infiltration, and also goblet cell hyperplasia. Conclusion: The results of this study revealed that BMSCs therapy significantly suppressed the lung pathology and inflammation in the ovalbumin induced asthma model in mouse.
引用
收藏
页码:55 / 63
页数:9
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