Multifunctional nanocomposites of superparamagnetic (Fe3O4) and NIR-responsive rare earth-doped up-conversion fluorescent (NaYF4 : Yb, Er) nanoparticles and their applications in biolabeling and fluorescent imaging of cancer cells

被引:147
作者
Mi, Congcong [1 ]
Zhang, Jingpu [1 ]
Gao, Huanyu [1 ]
Wu, Xianlong [1 ]
Wang, Meng [1 ]
Wu, Yingfan [3 ]
Di, Yueqin [3 ]
Xu, Zhangrun [3 ]
Mao, Chuanbin [2 ]
Xu, Shukun [1 ]
机构
[1] Northeastern Univ, Dept Chem, Shenyang 110004, Peoples R China
[2] Univ Oklahoma, Dept Chem & Biochem, Norman, OK 73019 USA
[3] Northeastern Univ, Res Ctr Analyt Sci, Shenyang 110004, Peoples R China
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
MAGNETIC-LUMINESCENT NANOCOMPOSITES; BIO-AFFINITY PROPERTIES; CORE/SHELL NANOCOMPOSITES; BIOMEDICAL APPLICATIONS; SHELL; CORE; NANOCRYSTALS; FABRICATION; SURFACE; DESIGN;
D O I
10.1039/c0nr00102c
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A new kind of magnetic/luminescent multifunctional nanoparticles was synthesized by covalently linking multiple carboxyl-functionalized superparamagnetic Fe3O4 nanoparticles and individual amino-functionalized silica-coated fluorescent NaYF4 : Yb, Er up-conversion nanoparticles (UCNPs). The resultant nanocomposites bear active carboxylic and amino groups on the surface that were proved to be chemically active and useful for further facile bioconjugation with biomolecules. The UCNPs in the nanocomposite particles can emit visible light in response to the irradiation by near infrared (NIR) light, enabling the application of the nanocomposites in bioimaging. X-Ray diffraction, infrared spectroscopy, transmission electron microscopy, luminescence spectroscopy, and magnetometry were applied to characterize the multifunctional nanocomposites. The nanocomposites exhibited good superparamagnetic and excellent green up-conversion photoluminescent properties that can be exploited in magnetic separation and guiding as well as bioimaging. Due to the presence of active functional groups on the nanocomposite surface, the Fe3O4/NaYF4 : Yb, Er magnetic/luminescent nanocomposites were successfully conjugated with a protein called transferrin, which specifically recognizes the transferrin receptors overexpressed on HeLa cells, and can be employed for biolabeling and fluorescent imaging of HeLa cells. Because NIR light can penetrate biological samples with good depth without damaging them and can avoid autofluorescence from them, the presence of both NIR-responsive UCNPs and superparamagnetic nanoparticles in the nanocomposite particles will enable the practical application of the nanocomposites in bioimaging and separation.
引用
收藏
页码:1141 / 1148
页数:8
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