The development of a highly selective fluorescent probe for the rapid detection of HClO in living cells and zebrafish

被引：4

作者：

Liang, Xing ^{[1
]}

Huo, Yonghui ^{[1
]}

Yan, Jun ^{[1
]}

Huang, Ling ^{[1
]}

Lin, Weiying ^{[1
]}

机构：

[1] Guangxi Univ, Guangxi Key Lab Electrochem Energy Mat, Inst Opt Mat & Chem Biol, Sch Chem & Chem Engn, Nanning 530004, Guangxi, Peoples R China

来源：

NEW JOURNAL OF CHEMISTRY | 2021年 / 45卷 / 28期

关键词：

HYPOCHLOROUS-ACID; EXCHANGE; WATER; SAMPLES;

D O I：

10.1039/d1nj00891a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Hypochlorous acid (HClO) is a significant signaling species which plays a decisive role in the immune system as an excellent defense against invasive bacteria. Therefore, it is significant to monitor HClO accurately and quickly in organisms. Herein, a new turn-on fluorescent probe (BM-HA) with a simple structure for rapid sensing of HClO is presented. BM-HA has favorable properties, including high selectivity, a large turn-on fluorescence signal (140-fold), and fast response (about 15 s). Additionally, quantum chemical calculations with DFT and TDDFT proved that reduced fluorescence of the BM-HA probe was possible due to the a-PET effect and the non-coplanarity of the coumarin and the benzothiazole. Furthermore, imaging in living cells and zebrafish indicates that the probe can potentially be used to detect HClO in complex biosystems.

引用

页码：12569 / 12575

页数：7