In recent years, the N, P in water bodies increased year by year, amount of water has been polluted seriously, it will damage the function of water body, reduce the value of water resources, limit the development of human beings. Three-phase biological fluidized bed with four step-feed has a good denitrification efficiency. Sludge reflux ratio, HRT, the concentration of dissolved oxygen and other factors have great influence on it. This article is a research these factors.