Simultaneous photometric microplate assay for free and total thiamine using gold nanoparticles and alkaline phosphatase

被引：10
作者：
Angeles Molina-Delgado, Maria [1 ]
Paz Aguilar-Caballos, Maria [1 ]
Gomez-Hens, Agustina [1 ]
机构：
[1] Univ Cordoba, Inst Fine Chem & Nanochem, Dept Analyt Chem, Campus Rabanales,Annex Marie Curie Bldg, E-14071 Cordoba, Spain
来源：
MICROCHIMICA ACTA | 2016年 / 183卷 / 04期
关键词：
Food analysis; Vitamin B1; Thiamine pyrophosphate; Enzymatic assay; Nanomaterial; Aggregation; SELECTIVE DETERMINATION; LIQUID-CHROMATOGRAPHY; ESTERS; VITAMIN-B1;
D O I：
10.1007/s00604-016-1767-x
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A photometric method is described for the determination of free and total thiamine (TH; vitamin B1) in food samples. It is based on the use of gold nanoparticles (AuNPs) which aggregate in the presence of TH owing to the interaction of its sulfur atom with the AuNPs. This is accompanied by a color change from wine red to purple-blue and an increase in absorbance at 590 nm. The effect is not observed for TH phosphates which, however, can be determined, as demonstrated for the example of TH pyrophosphate as a model ester, by treating them with alkaline phosphatase (ALP) which hydrolyses such esters. The use of two sample aliquots, one with and one without ALP, allows the determination of free and total TH, respectively. The dynamic range extends from 0.15 to 3.5 mu M, and the detection limit is 54 nM of TH. The method has been applied to the analysis of spiked food samples and gave recoveries that ranged between 88.8 and 100.7 %.
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收藏
页码：1385 / 1390
页数：6
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