A new plasmid-encoded proteic killer gene system: Cloning, sequencing, and analysing hig locus of plasmid Rts1

被引:69
|
作者
Tian, QB [1 ]
Ohnishi, M [1 ]
Tabuchi, A [1 ]
Terawaki, Y [1 ]
机构
[1] SHINSHU UNIV,SCH MED,DEPT BACTERIOL,MATSUMOTO,NAGANO 390,JAPAN
关键词
D O I
10.1006/bbrc.1996.0396
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A new proteic killer gene system, hig, was identified on the plasmid Rts1. The hig locus consisting of higA and higB is directly related to the temperature sensitive host cell growth conferred by Rts1. We proved that higB encoding presumably a 92-amino-acid polypeptide inhibited segregation of plasmid free cells, and higA encoding a 104-amino-acid polypeptide suppressed the HigB function both in cis and in trans. (C) 1996 Academic Press, Inc.
引用
收藏
页码:280 / 284
页数:5
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