Burkholderia phenoliruptrix sp nov., to accommodate the 2,4,5-trichlorophenoxyacetic acid and halophenol-degrading strain AC1100

被引:40
作者
Coenye, T
Henry, D
Speert, DP
Vandamme, P
机构
[1] State Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium
[2] Univ British Columbia, Dept Pediat, Div Infect Dis & Immunol, Vancouver, BC V5Z 1M9, Canada
关键词
Burkholderia phenoliruptrix; taxonomy; biodegradation; strain AC1100;
D O I
10.1078/0723202042369992
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Strain AC1100 is well-known for its ability to degrade a variety of recalcitrant xenobiotics, including 2,4,5-trichlorophenoxyacetic acid. We performed a polyphasic-taxonomic study to determine its taxonomic position. The G+C content of strain AC1100 was 62.6 mol%. On the basis of 16S rRNA gene sequence similarity, strain AC1100 belonged to the beta-Proteobacteria and was most closely related to Burkbolderia fungorum (98.3% similarity). DNA-DNA hybridisations, comparison of protein profiles, cellular fatty acid analysis and biochemical tests allowed genotypic and phenotypic differentiation of strain AC1100 from other Burkbolderia species. Our data show that strain AC1100 represents a novel species for which the name Burkbolderia phenoliruptrix sp. nov. is proposed. The type strain is AC1100(T) (= LX4G 22037(T) = CCUG 48558(T)).
引用
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页码:623 / 627
页数:5
相关论文
共 17 条
[1]  
[Anonymous], CHEM METHODS PROKARY
[2]   Enhancement of in vitro growth and resistance to gray mould of Vitis vinifera co-cultured with plant growth-promoting rhizobacteria [J].
Barka, EA ;
Belarbi, A ;
Hachet, C ;
Nowak, J ;
Audran, JC .
FEMS MICROBIOLOGY LETTERS, 2000, 186 (01) :91-95
[3]   Diversity and significance of Burkholderia species occupying diverse ecological niches [J].
Coenye, T ;
Vandamme, P .
ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (09) :719-729
[4]   Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. [J].
Coenye, T ;
Falsen, E ;
Vancanneyt, M ;
Hoste, B ;
Govan, JRW ;
Kersters, K ;
Vandamme, P .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 :405-413
[5]   Burkholderia fungorum sp nov, and Burkholderia caledonica sp nov., two new species isolated from the environment, animals and human clinical samples [J].
Coenye, T ;
Laevens, S ;
Willems, A ;
Ohlén, M ;
Hannant, W ;
Govan, JRW ;
Gillis, M ;
Falsen, E ;
Vandamme, P .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :1099-1107
[6]   FLUOROMETRIC DEOXYRIBONUCLEIC ACID DEOXYRIBONUCLEIC ACID HYBRIDIZATION IN MICRODILUTION WELLS AS AN ALTERNATIVE TO MEMBRANE-FILTER HYBRIDIZATION IN WHICH RADIOISOTOPES ARE USED TO DETERMINE GENETIC RELATEDNESS AMONG BACTERIAL STRAINS [J].
EZAKI, T ;
HASHIMOTO, Y ;
YABUUCHI, E .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (03) :224-229
[7]   Diversity of transconjugants that acquired plasmid pJP4 or pEMT1 after inoculation of a donor strain in the A- and B-horizon of an agricultural soil and description of Burkholderia hospita sp nov and Burkholderia terricola sp nov. [J].
Goris, J ;
Dejonghe, W ;
Falsen, E ;
De Clerck, E ;
Geeraerts, B ;
Willems, A ;
Top, EM ;
Vandamme, P ;
De Vos, P .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2002, 25 (03) :340-352
[8]   Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium [J].
Henry, DA ;
Campbell, ME ;
LiPuma, JJ ;
Speert, DP .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (03) :614-619
[9]   Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex [J].
Henry, DA ;
Mahenthiralingam, E ;
Vandamme, P ;
Coenye, T ;
Speert, DP .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (03) :1073-1078
[10]   METABOLISM OF HALOPHENOLS BY 2,4,5-TRICHLOROPHENOXYACETIC ACID DEGRADING PSEUDOMONAS-CEPACIA [J].
KARNS, JS ;
KILBANE, JJ ;
DUTTAGUPTA, S ;
CHAKRABARTY, AM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 46 (05) :1176-1181