Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells

被引:4
|
作者
Bu, Shuhan [1 ]
Nguyen, Hien C. [1 ,2 ]
Michels, David C. R. [1 ]
Rasheed, Berk [1 ,2 ]
Nikfarjam, Sepideh [1 ,2 ]
Singh, Rohan [1 ]
Wang, Lynn [1 ,2 ]
Patel, Darshil A. [1 ]
Singh, Shweta [1 ]
Qadura, Mohammad [3 ,4 ]
Singh, Krishna K. [1 ,2 ,5 ]
机构
[1] Univ Western Ontario, Schulich Sch Med & Dent, Dept Med Biophys, 1151 Richmond St N, London, ON N6A 5C1, Canada
[2] Univ Western Ontario, Anat & Cell Biol, Schulich Sch Med & Dent, London, ON, Canada
[3] St Michaels Hosp, Vasc Surg, Keenan Res Ctr Biomed Sci, Toronto, ON, Canada
[4] St Michaels Hosp, Li Ka Shing Knowledge Inst, Toronto, ON, Canada
[5] Univ Toronto, Pharmacol & Toxicol, Toronto, ON, Canada
基金
加拿大健康研究院;
关键词
angiotensin II; endothelial cell; lncRNA; GENOME-WIDE ASSOCIATION; FIBROBLAST-GROWTH-FACTOR; NITRIC-OXIDE; EXTRACELLULAR-MATRIX; BLOOD-PRESSURE; AT1; RECEPTOR; DNA-DAMAGE; EXPRESSION; PROTEIN; GENE;
D O I
10.1097/HJH.0000000000003140
中图分类号
R6 [外科学];
学科分类号
1002 ; 100210 ;
摘要
Objective: Angiotensin II (Ang II)-induced endothelial dysfunction plays an important role in the pathogenesis of cardiovascular diseases such as systemic hypertension, cardiac hypertrophy and atherosclerosis. Recently, long noncoding RNAs (lncRNAs) have been shown to play an essential role in the pathobiology of cardiovascular diseases; however, the effect of Ang II on lncRNAs and coding RNAs expression in endothelial cells has not been evaluated. Accordingly, we sought to evaluate the expression profiles of lncRNAs and coding RNAs in endothelial cells following treatment with Ang II. Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10(-6) mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0. Results: In HUVECs following Ang II treatment, from a total of 30 584 lncRNA targets screened, 25 targets were significantly upregulated, while 69 were downregulated. In the same HUVECs samples, from 26 106 mRNA targets screened, 28 targets were significantly upregulated and 67 were downregulated. Of the differentially expressed lncRNAs, RP11-354P11.2 and RP11-360F5.1 were the most upregulated (11-fold) and downregulated (three-fold) lncRNAs, respectively. Assigning the differentially regulated genes into functional groups using bioinformatics reveals numerous genes involved in the nucleotide excision repair and ECM-receptor interaction. Conclusion: This is the first study to profile the Ang II-induced differentially expressed lncRNAs and mRNAs in human endothelial cells. Our results reveal novel targets and substantially extend the list of potential candidate genes involved in Ang II-induced endothelial dysfunction and cardiovascular diseases.
引用
收藏
页码:1303 / 1313
页数:11
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