Retroperitoneal white adipose tissue lipoprotein lipase activity is rapidly down-regulated in response to acute stress

被引:9
作者
Casanovas, Albert [1 ]
Parramon, Nuria [1 ]
de la Cruz, Ferran [1 ]
Andres, Olga [1 ]
Terencio, Jose [1 ]
Lopez-Tejero, M. Dolores [1 ]
Llobera, Miquel [1 ]
机构
[1] Univ Barcelona, Fac Biol, Dept Bioquim & Biol Mol, E-08007 Barcelona, Spain
关键词
plasma; fight-or-flight; rat; immobilization; blood pressure; heart rate; IMMOBILIZATION STRESS; GENE-EXPRESSION; NITRIC-OXIDE; NEUROENDOCRINE RESPONSES; INTERMEDIATE METABOLISM; SKELETAL-MUSCLE; RAT ADIPOCYTES; PLASMA; ISOPROTERENOL; RELEASE;
D O I
10.1194/jlr.M600487-JLR200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tissue-specific regulation of LPL has been widely studied in rats. Previous studies reported that in vivo administration of adrenaline and acute stress cause an increase in plasma LPL activity coinciding with a decrease in white adipose tissue (WAT) LPL activity. We studied the speed of LPL activity changes during 30 min of stress by immobilization (IMMO) in rats. A first experimental approach in permanently cannulated rats permitted sequential blood sampling in the same animal during IMMO and the obtaining of hemodynamic parameters. In a second experimental approach, animals were euthanized at different times after the start of IMMO to determine LPL activity in tissues. Stress was characterized by rises in blood pressure, heart rate, plasma corticosterone, and available circulating energy substrates. Five min after the start of INMO, LPL activity fell in retroperitoneal WAT and increased in plasma. These data show the quickest LPL activity change ever described in response to a physiological situation. The speed and simultaneity of these changes suggest that the release from endothelium to the bloodstream may constitute a fast non-explored mechanism of tissue LPL activity regulation, involved in the lipid energy-substrate redistribution between tissues needed to prepare the "fight-or-flight" response.
引用
收藏
页码:863 / 868
页数:6
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