Frequent occurrence of multidrug-resistant CC17 Enterococcus faecium among clinical isolates in Sweden

被引:12
作者
Billstrom, H. [1 ]
Top, J. [2 ]
Edlund, C. [1 ]
Lund, B. [1 ,3 ]
机构
[1] Karolinska Inst, Karolinska Univ Hosp Huddinge, Div Clin Microbiol, Stockholm, Sweden
[2] Univ Med Ctr Utrecht, Dept Med Microbiol, Utrecht, Netherlands
[3] Karolinska Inst, Karolinska Univ Hosp Huddinge, Div Oral & Maxillofacial Surg, Stockholm, Sweden
关键词
antibiotic resistance; CC17; Enterococcus faecium; esp; hyl; multilocus sequence typing; TANDEM REPEAT ANALYSIS; ANTIBIOTIC-RESISTANCE; BIOFILM FORMATION; VIRULENCE GENE; TYPING SCHEME; ESP; EMERGENCE; SPREAD; HOSPITALS; HYL;
D O I
10.1111/j.1365-2672.2009.04585.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To screen for the globally spread cluster of Enterococcus faecium, clonal complex 17 (CC17) and characterize the genetic profile of Swedish clinical Ent. faecium isolates. Methods: A total of 203 consecutive isolates collected from 2004 to 2007 from patients with bacteraemia in Sweden. All isolates were genotyped using multiple-locus variable-number tandem repeat analysis (MLVA) and 20 isolates representing different MLVA types (MT) were chosen for multilocus sequence typing (MLST). Minimal inhibitory concentrations against clinically relevant antibiotics were determined with agar dilution. Presence of the virulence genes esp and hyl was investigated using PCR. Results: A total of 65% (n = 109) of all isolates belonged to MT-1, and the second most common MLVA type was MT-159 (13%, n = 21). MLST analysis confirmed the presence of CC17 during the entire study period. The number of isolates resistant to gentamicin and vancomycin, as well as the presence of hyl, increased significantly during the investigation period. Conclusions: The present study demonstrates that nosocomial infections caused by Ent. faecium CC17 are commonly occurring in Sweden. Significance and Impact of the Study: This is the first report of CC17 Ent. faecium in Sweden. The increase of antibiotic resistance and virulence indicates that these strains are further adapting to the hospital environment.
引用
收藏
页码:1810 / 1816
页数:7
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