Monitoring live cell viability: Comparative study of fluorescence, oblique incidence reflection and phase contrast microscopy imaging techniques

被引：18
作者：
Landry, S
McGhee, PL
Girardin, RJ
Keeler, WJ
机构：
[1] TBRHSC, Med Phys Program, Thunder Bay, ON P7B 6V4, Canada
[2] Lakehead Univ, Dept Phys, Thunder Bay, ON P7B 5E1, Canada
来源：
OPTICS EXPRESS | 2004年 / 12卷 / 23期
关键词：
D O I：
10.1364/OPEX.12.005754
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The relative performances of fluorescence, oblique incidence reflection and phase contrast imaging techniques have been studied for the purpose of monitoring long-term cellular activity and cell viability of several types of normal and cancerous cells in cultures. Time-lapse movies of live cell imaging of untagged and green fluorescent protein (GFP) tagged cell lines are presented. Oblique incidence reflection microscopy is the simplest and least expensive method to implement, appears to be the least phototoxic to cells, and is recommended for use in long-term optical monitoring of cell viability. (C) 2004 Optical Society of America.
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收藏
页码：5754 / 5759
页数：6
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