Knockdown of long noncoding RNA HUMT inhibits the proliferation and metastasis by regulating miR-455-5p/LRP4 axis in hepatocellular carcinoma

被引:6
作者
Zou, Xianzhi [1 ]
Sun, Peng [2 ]
Xie, Hui [3 ]
Fan, Lu [4 ]
Ding, Kun [3 ]
Wang, Jiyang [5 ]
Li, Yang [6 ,7 ]
机构
[1] Yantai Qishan Hosp, Dept Med Intervent Oncol, Yantai, Shandong, Peoples R China
[2] Yantai Qishan Hosp, Dept Med Gastroenterol, Yantai, Shandong, Peoples R China
[3] Yantai Qishan Hosp, Dept Internal Med, Yantai, Shandong, Peoples R China
[4] Yantai Qishan Hosp, Dept Liver Dis, Yantai, Shandong, Peoples R China
[5] Shandong Univ Chinese Med, Dept Phys Examinat Ctr, Affiliated Hosp 2, Jinan, Shandong, Peoples R China
[6] Shanxi Med Univ, Shanxi Bethune Hosp, Tongji Shanxi Hosp, Shanxi Acad Med Sci,Gen Med Dept,Hosp 3, Taiyuan 030032, Shanxi, Peoples R China
[7] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Wuhan, Hubei, Peoples R China
关键词
hepatic cancer; lncRNA HUMT; miR-455-5p; LRP4; RADIOFREQUENCY ABLATION; CELL-PROLIFERATION; HEPATIC CARCINOMA; LIVER-CANCER; EXPRESSION; MIGRATION; DIAGNOSIS;
D O I
10.1080/21655979.2022.2051841
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The present study aimed at investigating the effects and mechanism of long noncoding RNA highly upregulated in metastatic triple-negative breast cancer lymph node (lncRNA HUMT) in hepatocellular carcinoma (HCC). Quantitative real-time polymerase chain reaction was used to assess the expression of HUMT, microRNA (miR)-455-5p, and low-density lipoprotein receptor-related protein 4 (LRP4) in HCC tissues. Colony forming and 5-ethynyl-2 '-deoxyuridine assays were performed to assess cell proliferation. Transwell assay was performed to measure cell migration and invasion. Cell cycle distribution was assessed using flow cytometry. The protein expression of LRP4, proliferating cell nuclear antigen (PCNA), matrix metallopeptidase 2 (MMP-2), and MMP-9 was detected using western blot. Luciferase reporter assay and RNA immunoprecipitation assay was used to confirm the target association between miR-455-5p and HUMT or LRP4. In our study, the level of HUMT was enhanced in HCC tissues and cells. Cell proliferation, invasion, and migration in HCC cells were repressed by knockdown of HUMT, and knockdown of HUMT arrested cells in G1 phase and decreased the levels of PCNA, MMP-2, and MMP-9. MiR-455-5p was a target of HUMT. Lowexpression of miR-455-5p reversed the inhibitive influence on HCC cells induced by of HUMT silencing. LRP4 was a target of miR-455-5p and was negatively regulated by miR-455-5p. In addition, LRP4 expression was positively modified by HUMT, and LRP4 inhibited the inhibitory effects on HCC cells induced by HUMT silencing. In conclusion, HCC cell proliferation, invasion, and migration were restrained by knockdown of HUMT, which was related to the miR-455-5p/LRP4 axis.
引用
收藏
页码:8051 / 8063
页数:13
相关论文
共 44 条
[21]   Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 [J].
Love, Michael I. ;
Huber, Wolfgang ;
Anders, Simon .
GENOME BIOLOGY, 2014, 15 (12)
[22]   MicroRNA [J].
Lu, Thomas X. ;
Rothenberg, Marc E. .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2018, 141 (04) :1202-1207
[23]   Biological functions and clinical significance of the newly identified long non-coding RNA RP1-85F18.6 in colorectal cancer [J].
Ma, Yeshuo ;
Chen, Yifei ;
Lin, Changwei ;
Hu, Gui .
ONCOLOGY REPORTS, 2018, 40 (05) :2648-2658
[24]   LRP4 promotes migration and invasion of gastric cancer under the regulation of microRNA-140-5p [J].
Mao, Zhijun ;
Wang, Zhen ;
Zhang, Shiping ;
Pu, Yansong ;
Wang, Jianhua ;
Zhang, Tao ;
Long, Yanbin ;
Liu, Yi ;
Ma, Yu ;
Zhu, Jing .
CANCER BIOMARKERS, 2020, 29 (02) :245-253
[25]   MicroRNA-based therapeutics in cardiovascular disease: screening and delivery to the target [J].
Mellis, David ;
Caporali, Andrea .
BIOCHEMICAL SOCIETY TRANSACTIONS, 2018, 46 :11-21
[26]   Unique features of long non-coding RNA biogenesis and function [J].
Quinn, Jeffrey J. ;
Chang, Howard Y. .
NATURE REVIEWS GENETICS, 2016, 17 (01) :47-62
[27]   Blood exosomal micro ribonucleic acid profiling reveals the complexity of hepatocellular carcinoma and identifies potential biomarkers for differential diagnosis [J].
Sheng, Lang-Qing ;
Li, Jia-Rong ;
Qin, Hao ;
Liu, Ling ;
Zhang, Dong ;
Zhang, Qi ;
Huang, Meng-Li ;
Li, Xiao-Li ;
Xu, Xiao-Ya ;
Wei, Yang-Nian ;
Chen, Zi-Shuo ;
Luo, Hui ;
Zhang, Ji-Yang ;
Zhou, Cheng-Hui ;
Chen, Hao ;
Chen, Ze-Guo ;
Li, Fu-Gen ;
Li, Nian-Feng .
WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY, 2020, 12 (10) :1195-1208
[28]   Hepatocellular carcinoma in the era of immunotherapy [J].
Sim, Hao-Wen ;
Knox, Jennifer .
CURRENT PROBLEMS IN CANCER, 2018, 42 (01) :40-48
[29]   MicroRNA Key to Angiogenesis Regulation: miRNA Biology and Therapy [J].
Tiwari, Ankit ;
Mukherjee, Bratati ;
Dixit, Manjusha .
CURRENT CANCER DRUG TARGETS, 2018, 18 (03) :266-277
[30]   A comparison between radiofrequency ablation combined with transarterial chemoembolization and surgical resection in hepatic carcinoma: A meta-analysis [J].
Wang, Hongye ;
Liu, Yihai ;
Shen, Kangjie ;
Dong, Yuxiang ;
Sun, Jinyu ;
Shu, Yimei ;
Wan, Xiaojie ;
Ren, Xiaohan ;
Wei, Xiyi ;
Zhai, Bo .
JOURNAL OF CANCER RESEARCH AND THERAPEUTICS, 2019, 15 (07) :1617-1623