ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite

被引：106

作者：

Wu, Luling ^{[1
]}

Wang, Yang ^{[2
,3
]}

Weber, Maria ^{[1
]}

Liu, Liyuan ^{[1
]}

Sedgwick, Adam C. ^{[1
]}

Bull, Steven D. ^{[1
]}

Huang, Chusen ^{[2
,3
]}

James, Tony D. ^{[1
,4
]}

机构：

[1] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

[2] Shanghai Normal Univ, Educ Minist, Key Lab Resource Chem, Shanghai Key Lab Rare Earth Funct Mat, 100 Guilin Rd, Shanghai 200234, Peoples R China

[3] Shanghai Normal Univ, Shanghai Municipal Educ Comm, Key Lab Mol Imaging Probes & Sensors, Dept Chem, 100 Guilin Rd, Shanghai 200234, Peoples R China

[4] Sophia Univ, Fac Sci & Technol, Dept Mat & Life Sci, Chiyoda Ku, 7-1 Kioi Cho, Tokyo 1028554, Japan

来源：

CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 71期

关键词：

INTRAMOLECULAR PROTON-TRANSFER; ENDOPLASMIC-RETICULUM STRESS; STATE; SENSOR;

D O I：

10.1039/c8cc04919j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this work, we set out to develop an endoplasmic reticulum (ER) directed ESIPT-based ONOO- ratiometric fluorescent probe (ABAH-LW). ABAH-LW was synthesized in four steps and found to have a high sensitivity and selectivity towards the detection of ONOO-. ABAH-LW was able to detect low concentrations of ONOO- (limit of detection = 21.4 nM) within seconds producing a ratiometric change in fluorescence intensity. ABAH-LW further demonstrated the ability to ratiometrically image endogenous and exogenous ONOO- in HeLa cells. Moreover, co-localization experiments were carried out using commercially available ER-Tracker Red, Lyso-Tracker Red and Mito-Tracker-Red, which were co-stained with ABAH-LW in HeLa cells. For ER-Tracker Red, Pearson's correlation co-efficient of 0.93 was determined and 3D surface plot analysis illustrated a large overlap between ABAH-LW and ER-Tracker Red using both red and blue channels. In addition some co-localisation with Mito-Tracker Red and ABAH-LW was observed (0.73).

引用

页码：9953 / 9956

页数：4

共 26 条

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