Graphene Oxide Nanosheets Reshape Synaptic Function in Cultured Brain Networks

被引：119

作者：

Rauti, Rossana ^{[1
]}

Lozano, Neus ^{[2
,3
]}

Leon, Veronica ^{[4
]}

Scaini, Denis ^{[1
,5
]}

Musto, Mattia ^{[6
]}

Rago, Ilaria ^{[5
]}

Severino, Francesco P. Ulloa ^{[6
]}

Fabbro, Alessandra ^{[7
]}

Casalis, Loredana ^{[5
]}

Vazquez, Ester ^{[4
]}

Kostarelos, Kostas ^{[2
,3
]}

Prato, Maurizio ^{[7
,8
,9
]}

Ballerini, Laura ^{[1
,6
]}

机构：

[1] Univ Trieste, Dept Life Sci, I-34127 Trieste, Italy

[2] Univ Manchester, Sch Med, Nanomed Lab, Manchester M13 9PL, Lancs, England

[3] Univ Manchester, Fac Med & Human Sci, Natl Graphene Inst, Manchester M13 9PL, Lancs, England

[4] Univ Castilla La Mancha, Fac Ciencias & Tecnol Quim IRICA, Dept Quim Organ, E-13071 Ciudad Real, Spain

[5] ELETTRA Synchrotron Light Source, I-34149 Trieste, Italy

[6] Int Sch Adv Studies SISSA, I-34136 Trieste, Italy

[7] Univ Trieste, Dept Chem & Pharmaceut Sci, I-34127 Trieste, Italy

[8] CIC BiomaGUNE, Parque Tecnol San Sebastian, San Sebastian 20009, Guipuzcoa, Spain

[9] Ikerbasque, Basque Fdn Sci, Bilbao 48013, Spain

来源：

ACS NANO | 2016年 / 10卷 / 04期

基金：

英国惠康基金; 英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

nanotechnology; graphene; patch-clamp; synaptic terminals; exocytosis; FMI-43; microvesicles; CARBON NANOTUBES; MICROVESICLES; GLUTAMATE; CYTOTOXICITY; SYNAPSES; VESICLES; RELEASE; NANOMATERIALS; CHALLENGES; SCAFFOLDS;

D O I：

10.1021/acsnano.6b00130

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphene offers promising advantages for biomedical applications. However, adoption of graphene technology in biomedicine also poses important challenges in terms of understanding cell responses, cellular uptake, or the intracellular fate of soluble graphene derivatives. In the biological microenvironment, graphene nanosheets might interact with exposed cellular and subcellular structures, resulting in unexpected regulation of sophisticated biological signaling. More broadly, biomedical devices based on the design of these 2D planar nanostructures for interventions in the central nervous system require an accurate understanding of their interactions with the neuronal milieu. Here, we describe the ability of graphene oxide nanosheets to down-regulate neuronal signaling without affecting cell viability.

引用

页码：4459 / 4471

页数：13

共 66 条

[1] BDNF regulates spontaneous correlated activity at early developmental stages by increasing synaptogenesis and expression of the K+/Cl- co-transporter KCC2 [J].