Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls

被引:13
作者
Vale, Danniele L. [1 ]
Dias, Daniel S. [1 ]
Machado, Amanda S. [2 ]
Ribeiro, Patricia A. F. [1 ]
Lage, Daniela P. [1 ]
Costa, Lourena E. [1 ]
Steiner, Bethina T. [3 ]
Tavares, Grasiele S., V [1 ]
Ramos, Fernando F. [1 ]
Martinez-Rodrigo, Abel [4 ]
Chavez-Fumagalli, Miguel A. [1 ]
Caligiorne, Rachel B. [2 ]
De Magalhaes-Soares, Danielle F. [5 ]
Silveira, Julia A. G. [6 ]
Machado-de-Avila, Ricardo A. [3 ]
Teixeira, Antonio L. [1 ,7 ,8 ]
Coelho, Eduardo A. F. [1 ,9 ]
机构
[1] Univ Fed Minas Gerais, Fac Med, Programa Posgrad Ciencias Saude Infectol & Med Tr, BR-30130100 Belo Horizonte, MG, Brazil
[2] Santa Casa Belo Horizonte, Inst Ensino & Pesquisa, Rua Domingos Vieira 590, BR-30150240 Belo Horizonte, MG, Brazil
[3] Univ Extremo Sul Catarinense, Programa Posgrad Ciencias Saude, BR-88806000 Criciuma, SC, Brazil
[4] Univ Complutense Madrid, Fac Vet Sci, Dept Anim Hlth, Madrid 28040, Spain
[5] Univ Fed Minas Gerais, Escola Vet, Dept Med Vet Prevent, BR-31270901 Belo Horizonte, MG, Brazil
[6] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, BR-31270901 Belo Horizonte, MG, Brazil
[7] Univ Texas Hlth Sci Ctr Houston, McGovern Med Sch, Dept Psychiat & Behav Sci, Neuropsychiat Program, Houston, TX 77030 USA
[8] 1941 East Rd, Houston, TX 77041 USA
[9] Univ Fed Minas Gerais, COLTEC, Dept Patol Clin, BR-31270901 Belo Horizonte, MG, Brazil
关键词
Amastin; Recombinant proteins; Synthetic peptides; Diagnosis; Response immune; Visceral leishmaniasis; LINKED IMMUNOSORBENT ASSAYS; HYPOTHETICAL PROTEIN; A2; ANTIGEN; SERODIAGNOSIS; DOGS; DONOVANI; IDENTIFICATION; AMAZONENSIS; INFECTIONS; CHAGASI;
D O I
10.1016/j.diagmicrobio.2019.04.015
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The diagnosis of visceral leishmaniasis (VL) presents problems due to the toxicity and/or high cost of drugs. In addition, no vaccine exists to protect against human disease. In this study, the antigenicity and immunogenicity of amastin protein were evaluated in L. infantum-infected dogs and humans. For the diagnosis, besides the recombinant protein, 1 linear B-cell epitope was synthetized and evaluated in serological assays. Results showed high sensitivity and specificity values to detect the disease when both antigens were employed against a canine and human serological panel. By contrast, when using rA2 and a soluble Leishmania antigenic preparation, sensitivity and specificity values proved to be lower. A preliminary immunogenicity study showed that the amastin protein induced high IFN-gamma and low IL-10 production in stimulated PBMC derived from treated VL patients and healthy subjects, thus suggesting a potential use of this protein as an immunogen to protect against human disease. (C) 2019 Elsevier Inc. All rights reserved.
引用
收藏
页码:134 / 143
页数:10
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