Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls

被引：15

作者：

Vale, Danniele L. ^{[1
]}

Dias, Daniel S. ^{[1
]}

Machado, Amanda S. ^{[2
]}

Ribeiro, Patricia A. F. ^{[1
]}

Lage, Daniela P. ^{[1
]}

Costa, Lourena E. ^{[1
]}

Steiner, Bethina T. ^{[3
]}

Tavares, Grasiele S., V ^{[1
]}

Ramos, Fernando F. ^{[1
]}

Martinez-Rodrigo, Abel ^{[4
]}

Chavez-Fumagalli, Miguel A. ^{[1
]}

Caligiorne, Rachel B. ^{[2
]}

De Magalhaes-Soares, Danielle F. ^{[5
]}

Silveira, Julia A. G. ^{[6
]}

Machado-de-Avila, Ricardo A. ^{[3
]}

Teixeira, Antonio L. ^{[1
,7
,8
]}

Coelho, Eduardo A. F. ^{[1
,9
]}

机构：

[1] Univ Fed Minas Gerais, Fac Med, Programa Posgrad Ciencias Saude Infectol & Med Tr, BR-30130100 Belo Horizonte, MG, Brazil

[2] Santa Casa Belo Horizonte, Inst Ensino & Pesquisa, Rua Domingos Vieira 590, BR-30150240 Belo Horizonte, MG, Brazil

[3] Univ Extremo Sul Catarinense, Programa Posgrad Ciencias Saude, BR-88806000 Criciuma, SC, Brazil

[4] Univ Complutense Madrid, Fac Vet Sci, Dept Anim Hlth, Madrid 28040, Spain

[5] Univ Fed Minas Gerais, Escola Vet, Dept Med Vet Prevent, BR-31270901 Belo Horizonte, MG, Brazil

[6] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, BR-31270901 Belo Horizonte, MG, Brazil

[7] Univ Texas Hlth Sci Ctr Houston, McGovern Med Sch, Dept Psychiat & Behav Sci, Neuropsychiat Program, Houston, TX 77030 USA

[8] 1941 East Rd, Houston, TX 77041 USA

[9] Univ Fed Minas Gerais, COLTEC, Dept Patol Clin, BR-31270901 Belo Horizonte, MG, Brazil

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2019年 / 95卷 / 02期

关键词：

Amastin; Recombinant proteins; Synthetic peptides; Diagnosis; Response immune; Visceral leishmaniasis; LINKED IMMUNOSORBENT ASSAYS; HYPOTHETICAL PROTEIN; A2; ANTIGEN; SERODIAGNOSIS; DOGS; DONOVANI; IDENTIFICATION; AMAZONENSIS; INFECTIONS; CHAGASI;

D O I：

10.1016/j.diagmicrobio.2019.04.015

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The diagnosis of visceral leishmaniasis (VL) presents problems due to the toxicity and/or high cost of drugs. In addition, no vaccine exists to protect against human disease. In this study, the antigenicity and immunogenicity of amastin protein were evaluated in L. infantum-infected dogs and humans. For the diagnosis, besides the recombinant protein, 1 linear B-cell epitope was synthetized and evaluated in serological assays. Results showed high sensitivity and specificity values to detect the disease when both antigens were employed against a canine and human serological panel. By contrast, when using rA2 and a soluble Leishmania antigenic preparation, sensitivity and specificity values proved to be lower. A preliminary immunogenicity study showed that the amastin protein induced high IFN-gamma and low IL-10 production in stimulated PBMC derived from treated VL patients and healthy subjects, thus suggesting a potential use of this protein as an immunogen to protect against human disease. (C) 2019 Elsevier Inc. All rights reserved.

引用

页码：134 / 143

页数：10

共 53 条

[21] Comparison of serological assays for the diagnosis of canine visceral leishmaniasis in animals presenting different clinical manifestations [J].