Analysis of the 5′-flanking regions of the MHC-linked Hsp70-2 and Hsp70-3 genes of the rat

被引：5

作者：

Friedrich, H ^{[1
]}

Walter, L ^{[1
]}

Günther, E ^{[1
]}

机构：

[1] Univ Gottingen, Div Immunogenet, D-37073 Gottingen, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1395卷 / 01期

关键词：

Hsp70; MHC; gene regulation;

D O I：

10.1016/S0167-4781(97)00154-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have analyzed the genomic interval between the MHC-linked heat shock protein 70 (Hsp70) genes Hsp70-2 and Hsp70-3 in the rat. The distance between the transcription start sites of both genes which are organized in a head-to-head orientation is 604 bp. Unlike Hsp70-2 the 5' untranslated region of Hsp70-3 is interrupted by an intron of about 3.9 kb. The Hsp70-3 promoter lacks a TATA box and heat shock elements, but contains two CRE elements, one Sp1-GC and two PuF binding sites. Analysis of the Hsp70-2 promoter by CAT assays reveals positive and negative regulatory elements extending into the Hsp70-3 gene. (C) 1998 Elsevier Science B.V.

引用

页码：57 / 61

页数：5

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