Electroacupuncture ameliorates experimental colitis induced by TNBS through activation of interleukin-10 and inhibition of iNOS in mice

被引：21

作者：

Araujo de Macedo Goes, Annya Costa ^{[1
]}

Martins Pinto, Francisco Maxwell ^{[1
]}

Fernandes, Guilherme Cardoso ^{[1
]}

Barbosa, Jessica Sales ^{[1
]}

Correia, Emanuela Santos ^{[1
]}

Ribeiro, Ronaldo Albuquerque ^{[1
]}

Guimaraes, Sergio Botelho ^{[1
]}

Pereira Lima Junior, Roberto Cesar ^{[1
]}

de Castro Brito, Gerly Anne ^{[1
]}

Rodrigues, Lusmar Veras ^{[1
]}

机构：

[1] Univ Fed Ceara, Dept Physiol & Pharmacol, Lab Pharmacol Inflammat & Canc LAFICA, Fortaleza, Ceara, Brazil

来源：

ACTA CIRURGICA BRASILEIRA | 2014年 / 29卷 / 12期

关键词：

Electroacupuncture; Inflammation; Colitis; Mice; ADRENOCORTICAL HORMONE PRODUCTION; ULCERATIVE-COLITIS; ACUPUNCTURE; INFLAMMATION; STIMULATION; MODEL; ACID; RATS;

D O I：

10.1590/S0102-86502014001900004

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

PURPOSE: To study the anti-inflammatory actions of electroacupuncture (EAc) on an experimental colitis model in mice. METHODS: Thirty-eight male Swiss mice, divided in five groups, were subjected to induction of colitis by TNBS in 50% ethanol. Saline (SAL) and ethanol (ETNL) groups served as controls. TNBS+EAc and TNBS+dexamethasone subgroups were treated with EAc 100Hz and dexamethasone (DEXA) 1 mg/Kg/day, respectively. After three days, a colon segment was obtained for quantification of myeloperoxidase (MPO) activity, immunohistochemistry for iNOS, malondialdehyde (MDA) and cytokines (IL-1 beta and IL-10). RESULTS: Neutrophilic activity, assayed as MPO activity, was significantly higher in the TNBS colitis group than that in the saline control group. TNBS+EAc group showed suppression of IL-10 in the colon. EAc treatment significantly reduced the concentration of MDA and the expression of iNOS, as compared to the other groups. CONCLUSION: Electroacupuncture 100Hz applied to acupoint ST-36 promotes an anti-inflammatory action on the TNBS-induced colitis, mediated by increase of IL-10 and decrease of iNOS expression.

引用

页码：787 / 793

页数：7

共 18 条

[1] Toll-like receptor 4 signaling leads to neutrophil migration impairment in polymicrobial sepsis [J].