Quantification of insulin in dried blood spots

被引：13

作者：

Dowlati, B ^{[1
]}

Dunhardt, PA ^{[1
]}

Smith, MM ^{[1
]}

Shaheb, S ^{[1
]}

Stuart, CA ^{[1
]}

机构：

[1] Univ Texas, Med Branch, Dept Internal Med, Div Endocrinol, Galveston, TX 77555 USA

来源：

JOURNAL OF LABORATORY AND CLINICAL MEDICINE | 1998年 / 131卷 / 04期

关键词：

D O I：

10.1016/S0022-2143(98)90188-3

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A method has been developed in which the concentrations of insulin in dried blood spots on filter paper can be estimated. The technique involves elution of blood from the paper, evaporation, and rehydration in assay buffer. The resuspended samples are then assayed by radioimmunoassay. The ratio of control plasma to control dried blood spot insulin values is used as a conversion factor for unknown samples on paper to express the dried blood spot insulin concentration in plasma equivalents. Intra-assay variability was 14%, and interassay variability was 25%, but the elution conversion factor had a variability of about 40% among individuals. This technique has substantial advantages for field studies, but elevated values should be confirmed by a venous plasma sample measurement in a conventional radioimmunoassay.

引用

页码：370 / 374

页数：5