Dominant negative stat3 mutant inhibits interleukin-6-induced Jak-STAT signal transduction

被引：202

作者：

Kaptein, A ^{[1
]}

Paillard, V ^{[1
]}

Saunders, M ^{[1
]}

机构：

[1] LABS GLAXO WELLCOME, ZA COURTABOEUF, CTR RECH, F-91951 LES ULIS, FRANCE

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1996年 / 271卷 / 11期

关键词：

D O I：

10.1074/jbc.271.11.5961

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interleukin-6 (IL-6) induces tyrosine phosphorylation and activation of the latent transcription factor Stat3 in HepG2 cells. Mutation of Stat3 tyrosine 705 to phenylalanine (Y705F) inhibits IL-6-induced tyrosine phosphorylation of this Stat3 mutant in transfected HepG2 cells. In cotransfections of HepG2 cells, the Stat3 mutant Y705F causes a reduction of the tyrosine phosphorylation of wild type Stat3-FLAG. Moreover, Y705F inhibits the action of endogenous Stat3 in cotransfected cells, reducing IL-6 induction of a Stat3-responsive reporter construct. Y705F therefore acts as a dominant negative mutation of Stat3.

引用

页码：5961 / 5964

页数：4

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