Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation

被引：307

作者：

Legras, JL ^{[1
]}

Karst, F ^{[1
]}

机构：

[1] INRA, Unite Mixte Rech Vigne & Vins Alsace, F-68021 Colnar, France

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 221卷 / 02期

关键词：

wine; molecular typing; genetic variability; delta element; Saccharomyces cerevisiae;

D O I：

10.1016/S0378-1097(03)00205-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new primer pair (delta12-delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：249 / 255

页数：7

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