Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation

被引：298
作者：
Legras, JL [1 ]
Karst, F [1 ]
机构：
[1] INRA, Unite Mixte Rech Vigne & Vins Alsace, F-68021 Colnar, France
来源：
FEMS MICROBIOLOGY LETTERS | 2003年 / 221卷 / 02期
关键词：
wine; molecular typing; genetic variability; delta element; Saccharomyces cerevisiae;
D O I：
10.1016/S0378-1097(03)00205-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A new primer pair (delta12-delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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页码：249 / 255
页数：7
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