The effect of osteoblasts on the surface oxidation processes of biodegradable Mg and Mg-Ag alloys studied by synchrotron IR microspectroscopy

被引：21

作者：

Agha, Nezha Ahmad ^{[1
]}

Liu, Zhidan ^{[1
]}

Feyerabend, Frank ^{[1
]}

Willumeit-Roemer, Regine ^{[1
]}

Gasharova, Billiana ^{[2
]}

Heidrich, Stefanie ^{[3
]}

Mihailova, Boriana ^{[3
]}

机构：

[1] Helmholtz Zentrum Geesthacht, Inst Mat Res, Max Planck Str 1, D-21502 Geesthacht, Germany

[2] Karlsruher Inst Technol, Inst Beschleunigerphys & Technol, POB 3640, D-76021 Karlsruhe, Germany

[3] Univ Hamburg, Dept Earth Sci, Grindelallee 48, D-20146 Hamburg, Germany

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2018年 / 91卷

关键词：

Mg; Degradable bone implants; Synchrotron FTIR spectroscopy; EMPA; IN-VIVO; MAGNESIUM DEGRADATION; CALCIUM-PHOSPHATE; BONE; CORROSION; OSTEOCLASTS; IMPLANTS; CATHETER; BEHAVIOR; LAE442;

D O I：

10.1016/j.msec.2018.06.001

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

High-resolution analytical methods, including synchrotron infrared microspectroscopy combined with wave-length-dispersive X-ray emission spectroscopy were applied to study the structure and chemical composition of the oxidized layer of pure and Ag-alloyed Mg exposed to cell culture media without and with osteoblasts. Comparative analysis has been done on pure Mg immersed in two different cell culture media: Dulbecco's Modified Eagles Medium (DMEM) and Roswell Park Memorial Institute medium (RPMI), whereas Mg-xAg binary alloys (x = 2, 4, 6, 8 wt%) were studied after immersion in DMEM. It is shown that the physicochemical formation of degradation products as well as the activity of the biological component is influenced by the addition of silver. It could be demonstrated that the presence of Ag in the Mg alloy enhances the chemical reaction between Mg and C to form amorphous and/or crystalline MgCO3 on account of CaCO3. As a consequence, the further available P and Ca react easily to form Mg-poor amorphous calcium phosphate phases. Osteoblasts actively adjusted these phases towards hydroxyapatite-like phases.

引用

页码：659 / 668

页数：10

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