Isolation, structural characterization, and lymphopoiesis stimulant activity of a polysaccharide from the abalone gonad

被引：20

作者：

Yang, Jing-feng ^{[1
]}

Li, Yue-hui ^{[1
]}

Zhao, Jun ^{[1
,2
]}

Li, Peng-fei ^{[1
]}

Zhu, Ce ^{[1
]}

Song, Ye-han ^{[1
]}

Zhang, Lan-yi ^{[1
]}

Zhu, Bei-wei ^{[1
]}

机构：

[1] Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, Dalian 116034, Peoples R China

[2] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2015年 / 24卷 / 01期

基金：

国家高技术研究发展计划(863计划);

关键词：

abalone; gonad; polysaccharide; backbone; lymphocyte; PROLIFERATION; METHYLATION; SULFATE;

D O I：

10.1007/s10068-015-0004-4

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A novel polysaccharide (AGP-32) from the gonad of Haliotis discus hannai Ino was isolated using a protease-assisted process and successive ion-exchange and gel-filtration chromatography. The backbone of AGP-32 was determined using hydrolysis with trifluoroacetic acid. FTIR, NMR, and methylation analysis, and periodate oxidation and Smith degradation analysis revealed that the AGP-32 backbone mainly consisted of (1 -> 6)-linked mannose, (1 -> 3)-linked galactose, and (1 -> 3)-linked glucose in a proportion of 2:3:1. An in vitro cell assay indicated that AGP-32 promoted mice splenic lymphocyte proliferation by 26% at a concentration of 50 mu g/mL. AGP-32 had an effect on immune protection and is a candidate for consideration as a functional food.

引用

页码：23 / 30

页数：8

共 25 条

[1] EVIDENCE FOR THYMUS-INDEPENDENT HUMORAL ANTIBODY PRODUCTION IN MICE AGAINST POLYVINYLPYRROLIDONE AND E-COLI LIPOPOLYSACCHARIDE [J].