Genomic insights into the circulation of pandemic fluoroquinolone-resistant extra-intestinal pathogenic Escherichia coli ST1193 in Vietnam

被引:7
|
作者
Nguyen, Quynh [1 ]
Nguyen, To Thi Nguyen [1 ]
Pham, Phuong [1 ]
Chau, Vinh [1 ]
Nguyen, Lan Phu Huong [2 ]
Nguyen, Toan Duc [3 ]
Ha, Tuyen Thanh [1 ]
Le, Nhi Thi Quynh [4 ]
Vu, Duong Thuy [3 ]
Baker, Stephen [5 ]
Thwaites, Guy E. [1 ,6 ]
Rabaa, Maia A. [1 ,6 ]
Pham, Duy Thanh [1 ,6 ]
机构
[1] Oxford Univ Clin Res Unit, Ho Chi Minh City, Vietnam
[2] Hosp Trop Dis, Ho Chi Minh City, Vietnam
[3] Childrens Hosp 1, Ho Chi Minh City, Vietnam
[4] Univ Med & Pharm, Ho Chi Minh City, Vietnam
[5] Univ Cambridge, Cambridge Inst Therapeut Immunol & Infect Dis CITI, Dept Med, Cambridge, England
[6] Univ Oxford, Ctr Trop Med & Global Hlth, Nuffield Dept Med, Oxford, England
来源
MICROBIAL GENOMICS | 2021年 / 17卷 / 12期
基金
英国惠康基金;
关键词
ExPEC; E; coli; ST1193; bloodstream infections; fluoroquinolone resistance;
D O I
10.1099/mgen.0.000733
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Extra-intestinal pathogenic Escherichia coli (ExPEC) ST1193, a globally emergent fluoroquinolone-resistant clone, has become an important cause of bloodstream infections (BSIs) associated with significant morbidity and mortality. Previous studies have reported the emergence of fluoroquinolone-resistant ExPEC ST1193 in Vietnam; however, limited data exist regarding the genetic structure, antimicrobial resistance (AMR) determinants and transmission dynamics of this pandemic clone. Here, we performed genomic and phylogenetic analyses of 46 ST1193 isolates obtained from BSIs and healthy individuals in Ho Chi Minh City, Vietnam, to investigate the pathogen population structure, molecular mechanisms of AMR and potential transmission patterns. We further examined the phylogenetic structure of ST1193 isolates in a global context. We found that the endemic E. coli ST1193 population was heterogeneous and highly dynamic, largely driven by multiple strain importations. Several well -supported phylogenetic clusters (C1-C6) were identified and associated with distinct blaCTX-M variants, including blaCTXM-27 (C1-C3, C5), blaCTXM-55 (C4) and blaCTXM-15 (C6). Most ST1193 isolates were multidrug-resistant and carried an extensive array of AMR genes. ST1193 isolates also exhibited the ability to acquire further resistance while circulating in Vietnam. There were phy-logenetic links between ST1193 isolates from BSIs and healthy individuals, suggesting these organisms may both establish long -term colonization in the human intestinal tract and induce infections. Our study uncovers factors shaping the population structure and transmission dynamics of multidrug-resistant ST1193 in Vietnam, and highlights the urgent need for local One Health genomic surveillance to capture new emerging ExPEC clones and to better understand the origins and transmission patterns of these pathogens.
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页数:10
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