A longstanding conjecture on biharmonic submanifolds, proposed by Chen in 1991, is that any biharmonic submanifold in a Euclidean space is minimal. In the case of a hypersurface M-n in Rn+1, Chen's conjecture was settled in the case of n = 2 by Chen and Jiang around 1987 independently. Hasanis and Vlachos in 1995 settled Chen's conjecture for a hypersurface with n = 3. However, the general Chen's conjecture on a hypersurface M-n remains open for n > 3. In this paper, we settle Chen's conjecture for hypersurfaces in R-5 for n = 4. (C) 2021 Elsevier Inc. All rights reserved.