ISOLATION AND CHARACTERIZATION OF MICROSATELLITE MARKERS IN BEILSCHMIEDIA ROXBURGHIANA (LAURACEAE)

被引：3

作者：

Liu, Lu ^{[1
,2
]}

Wang, Zheng-Feng ^{[3
]}

Li, Qiao-Ming ^{[1
]}

Ye, Wan-Hui ^{[3
]}

Cao, Hong-Lin ^{[3
]}

Wang, Zhang-Ming ^{[3
]}

机构：

[1] Chinese Acad Sci, Lab Plant Phylogenet & Conservat, Xishuangbanna Trop Bot Garden, Kunming 650223, Yunnan, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

[3] Chinese Acad Sci, Key Lab Vegetat Restorat & Management Degraded Ec, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China

来源：

APPLICATIONS IN PLANT SCIENCES | 2013年 / 1卷 / 08期

关键词：

Beilschmiedia roxburghiana; genetic diversity; Lauraceae; microsatellite markers; population genetics; Xishuangbanna Plot;

D O I：

10.3732/apps.1200549

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: Although there are as many as 250 species in the genus Beilschmiedia, their genetic diversity has been poorly investigated. Our objective was to develop microsatellite markers for B. roxburghiana to study its genetic diversity for the sustainable management of this species. Methods and Results: Using the microsatellite-enriched library and PCR-based screening method, 22 microsatellite markers were developed and 10 showed high polymorphism in a population. The number of alleles per locus for these 10 microsatellites ranged from five to 19. The observed and expected heterozygosities ranged from 0.298 to 1.000 and from 0.314 to 0.878, respectively. Conclusions: Our results from the 10 highly polymorphic microsatellites indicate that the principal reproductive mode of B. roxburghiana is clonal in the studied population. These microsatellites will facilitate further studies on genetic diversity and structure in B. roxburghiana

引用

页数：3

共 10 条

[1]

[Anonymous], 1991, MOL TECHNIQUES TAXON

[2] Tackling the population genetics of clonal and partially clonal organisms [J].