Transient β-glucuronidase expression in lily (Lilium longflorum L.) pollen via wounding-assisted Agrobacterium-mediated transformation

被引：15

作者：

Kim, Su-Sung ^{[1
]}

Shin, Dong-Il ^{[1
]}

Park, Hee-Sung ^{[1
]}

机构：

[1] Catholic Univ Daegu, Dept Biotechnol, Kyungsan 712702, Kyungbuk, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 06期

关键词：

aluminum oxide; lily pollen; transient expression; wounding;

D O I：

10.1007/s10529-007-9326-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A pollen-based transient expression system has been developed. Lily pollen grains, wounded by vigorous shaking in the presence of aluminum oxide particles, were transformed by infiltration with Agrobacterium tumefaciens LBA4404 cells harboring the beta-glucuronidase (GUS) gene construct, pBI121. In histochemical and fluorometric GUS analysis, the wounding processes allowed efficient transformation and, in cDNA blot hybridization, GUS mRNA synthesis was clearly detected. Lily pollen with appropriate wounds, therefore, can be used conveniently for the rapid production of recombinant proteins.

引用

页码：965 / 969

页数：5

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