A simple and sensitive fluorescent assay for hemin detection based on artemisinin-thiamine

被引：22
作者：
Ni, Pengjuan [1 ]
Chen, Chuanxia [1 ]
Jiang, Yuanyuan [1 ]
Lu, Yizhong [1 ]
Chen, Wei [2 ]
机构：
[1] Univ Jinan, Sch Mat Sci & Engn, Jinan, Shandong, Peoples R China
[2] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun, Jilin, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 273卷
基金：
中国国家自然科学基金;
关键词：
Hemin; Artemisinin; Thiamine; Fluorescence; INJECTION CHEMILUMINESCENCE DETERMINATION; LABEL-FREE; SELECTIVE DETECTION; HYDROGEN-PEROXIDE; QUANTUM DOTS; SENSOR; PROBE; MITOCHONDRIA; APTASENSOR; REDUCTION;
D O I：
10.1016/j.snb.2018.06.052
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Hemin, the most common biological iron form, serves many biological functions. However, studies on hemin detection are scarce. As an unusual natural peroxide, artemisinin is quite stable in the presence of common species. While, it can decompose to generate superoxide radicals in the presence of hemin. And the as-obtained superoxide radicals can effectively oxidize thiamine to form fluorescent thiochrome. Inspired by this, a simple and novel fluorescent assay for hemin detection based on artemisin-thiamine is developed. Based on the linear relationship between the fluorescence intensity and concentration of hemin, the developed artemisinin-thiamine assay can be utilized for sensitive detection of hemin in the concentration range of 2.0-300.0 nM with a detection limit of 0.68 nM. Moreover, this assay shows high selectivity over other cations and biomolecules. Besides, hemin can be detected in serum samples with satisfactory results, which demonstrates its great potential for real sample analysis. The designed assay is free of any fluorescent nanomaterials or probes by just mixing these reagents together, and all the detection processes is performed within 20 min. Therefore, it can offer a simple, highly sensitive and selective sensing platform for fluorescence detection of hemin.
引用
收藏
页码：198 / 203
页数：6
相关论文
共 27 条
  • [1] Evidence for hemin inducing the cleavage of peroxide bond of artemisinin (Qinghaosu): cyclic voltammetry and in situ FT IR spectroelectrochemical studies on the reduction mechanism of artemisinin in the presence of hemin
    Chen, Y
    Zheng, JM
    Zhu, SM
    Chen, HY
    [J]. ELECTROCHIMICA ACTA, 1999, 44 (14) : 2345 - 2350
  • [2] Flow injection chemiluminescence determination of thiamine based on its enhancing effect on the luminol-hydrogen peroxide system
    Du, JX
    Li, YH
    Lu, JR
    [J]. TALANTA, 2002, 57 (04) : 661 - 665
  • [3] High-Throughput Detection of Thiamine Using Periplasmic Binding Protein-Based Biorecognition
    Edwards, Katie A.
    Seog, Woo Jin
    Han, Lu
    Feder, Seth
    Kraft, Clifford E.
    Baeumner, Antje J.
    [J]. ANALYTICAL CHEMISTRY, 2016, 88 (16) : 8248 - 8256
  • [4] A carboxylated graphene and aptamer nanocomposite-based aptasensor for sensitive and specific detection of hemin
    Gao, Ling
    Xiao, Yinghong
    Wang, Yanping
    Chen, Xue
    Zhou, Bo
    Yang, Xiaodi
    [J]. TALANTA, 2015, 132 : 215 - 221
  • [5] Artemisinin-Luminol Chemiluminescence for Forensic Bloodstain Detection Using a Smart Phone as a Detector
    Gao, Wenyue
    Wang, Chao
    Muzyka, Kateryna
    Kitte, Shimeles Addisu
    Li, Jianping
    Zhang, Wei
    Xu, Guobao
    [J]. ANALYTICAL CHEMISTRY, 2017, 89 (11) : 6161 - 6166
  • [6] CHEMILUMINESCENT DETECTION OF ARTEMISININ - NOVEL ENDOPEROXIDE ANALYSIS USING LUMINOL WITHOUT HYDROGEN-PEROXIDE
    GREEN, MD
    MOUNT, DL
    TODD, GD
    CAPOMACCHIA, AC
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1995, 695 (02) : 237 - 242
  • [7] Heparin-MPA dual modified CdS quantum dots used as a simple and rapid label-free fluorescent sensor for protamine and hemin detection
    Guan, Guimei
    Sha, Jichao
    Zhu, Dongdong
    [J]. MICROCHEMICAL JOURNAL, 2017, 133 : 391 - 397
  • [8] Thiamine biosensor based on oxidative trapping of enzyme-substrate intermediate
    Halma, Matilte
    Doumeche, Bastien
    Hecquet, Laurence
    Prevot, Vanessa
    Mousty, Christine
    Charmantray, Franck
    [J]. BIOSENSORS & BIOELECTRONICS, 2017, 87 : 850 - 857
  • [9] Employing aqueous CdTe quantum dots with diversified surface functionalities to discriminate between heme (Fe(II)) and hemin (Fe(III))
    Han, Jishu
    Zhou, Ziwei
    Bu, Xinyuan
    Zhu, Shoujun
    Zhang, Hao
    Sun, Haizhu
    Yang, Bai
    [J]. ANALYST, 2013, 138 (12) : 3402 - 3408
  • [10] Flow injection chemiluminescence determination of hemin using the rhodamine B-H2O2-NaOH system
    Han, SQ
    Liu, EB
    Li, H
    [J]. MICROCHIMICA ACTA, 2005, 149 (3-4) : 281 - 286
123