Luciferase-induced photoreductive uncaging of small-molecule effectors

被引：37

作者：

Lindberg, Eric ^{[1
]}

Angerani, Simona ^{[1
]}

Anzola, Marcello ^{[1
]}

Winssinger, Nicolas ^{[1
]}

机构：

[1] Univ Geneva, Fac Sci, NCCR Chem Biol, Dept Organ Chem, 30 Quai Ernest Ansermet, CH-1211 Geneva, Switzerland

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

基金：

瑞士国家科学基金会;

关键词：

SELECTIVE TREATMENT; CANCER; FLUOROPHORE;

D O I：

10.1038/s41467-018-05916-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bioluminescence resonance energy transfer (BRET) is extensively used to study dynamic systems and has been utilized in sensors for studying protein proximity, metabolites, and drug concentrations. Herein, we demonstrate that BRET can activate a ruthenium-based photocatalyst which performs bioorthogonal reactions. BRET from luciferase to the ruthenium photocatalyst is used to uncage effector molecules with up to 64 turnovers of the catalyst, achieving concentrations >0.6 mu M effector with 10 nM luciferase construct. Using a BRET sensor, we further demonstrate that the catalysis can be modulated in response to an analyte, analogous to allosterically controlled enzymes. The BRET-induced reaction is used to uncage small-molecule drugs (ibrutinib and duocarmycin) at biologically effective concentrations in cellulo.

引用

页数：9

共 30 条

[1] Dual Readout BRET/FRET Sensors for Measuring Intracellular Zinc [J].

Aper, Stijn J. A. ;

Dierickx, Pieterjan ;

Merkx, Maarten .

ACS CHEMICAL BIOLOGY, 2016, 11 (10) :2854-2864

[2] Luminopsins integrate opto- and chemogenetics by using physical and biological light sources for opsin activation [J].

Berglund, Ken ;

Clissold, Kara ;

Li, Haofang E. ;

Wen, Lei ;

Park, Sung Young ;

Gleixner, Jan ;

Klein, Marguerita E. ;

Lu, Dongye ;

Barter, Joseph W. ;

Rossi, Mark A. ;

Augustine, George J. ;

Yin, Henry H. ;

Hochgeschwender, Ute .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (03) :E358-E367

[3] Light-Emitting Channelrhodopsins for Combined Optogenetic and Chemical-Genetic Control of Neurons [J].

Berglund, Ken ;

Birkner, Elisabeth ;

Augustine, George J. ;

Hochgeschwender, Ute .

PLOS ONE, 2013, 8 (03)

[4] Critical Analysis of Rate Constants and Turnover Frequency in Nucleic Acid-Templated Reactions: Reaching Terminal Velocity [J].

Chang, Dalu ;

Lindberg, Eric ;

Winssinger, Nicolas .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2017, 139 (04) :1444-1447

[5] A biomolecule-compatible visible-light-induced azide reduction from a DNA-encoded reaction-discovery system [J].

Chen, Yiyun ;

Kamlet, Adam S. ;

Steinman, Jonathan B. ;

Liu, David R. .

NATURE CHEMISTRY, 2011, 3 (02) :146-153

[6] Chronic active B-cell-receptor signalling in diffuse large B-cell lymphoma [J].

Davis, R. Eric ;

Ngo, Vu N. ;

Lenz, Georg ;

Tolar, Pavel ;

Young, Ryan M. ;

Romesser, Paul B. ;

Kohlhammer, Holger ;

Lamy, Laurence ;

Zhao, Hong ;

Yang, Yandan ;

Xu, Weihong ;

Shaffer, Arthur L. ;

Wright, George ;

Xiao, Wenming ;

Powell, John ;

Jiang, Jian-Kang ;

Thomas, Craig J. ;

Rosenwald, Andreas ;

Ott, German ;

Muller-Hermelink, Hans Konrad ;

Gascoyne, Randy D. ;

Connors, Joseph M. ;

Johnson, Nathalie A. ;

Rimsza, Lisa M. ;

Campo, Elias ;

Jaffe, Elaine S. ;

Wilson, Wyndham H. ;

Delabie, Jan ;

Smeland, Erlend B. ;

Fisher, Richard I. ;

Braziel, Rita M. ;

Tubbs, Raymond R. ;

Cook, J. R. ;

Weisenburger, Dennis D. ;

Chan, Wing C. ;

Pierce, Susan K. ;

Staudt, Louis M. .

NATURE, 2010, 463 (7277) :88-U97

[7] Bioluminescence resonance energy transfer (BRET) imaging of protein-protein interactions within deep tissues of living subjects [J].

Dragulescu-Andrasi, Anca ;

Chan, Carmel T. ;

De, Abhijit ;

Massoud, Tarik F. ;

Gambhir, Sanjiv S. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (29) :12060-12065

[8] Fast and high resolution single-cell BRET imaging [J].

Goyet, Elise ;

Bouquier, Nathalie ;

Ollendorff, Vincent ;

Perroy, Julie .

SCIENTIFIC REPORTS, 2016, 6

[9]

Griss R, 2014, NAT CHEM BIOL, V10, P598, DOI [10.1038/nchembio.1554, 10.1038/NCHEMBIO.1554]

[10] Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate [J].

Hall, Mary P. ;

Unch, James ;

Binkowski, Brock F. ;

Valley, Michael P. ;

Butler, Braeden L. ;

Wood, Monika G. ;

Otto, Paul ;

Zimmerman, Kristopher ;

Vidugiris, Gediminas ;

Machleidt, Thomas ;

Robers, Matthew B. ;

Benink, Helene A. ;

Eggers, Christopher T. ;

Slater, Michael R. ;

Meisenheimer, Poncho L. ;

Klaubert, Dieter H. ;

Fan, Frank ;

Encell, Lance P. ;

Wood, Keith V. .

ACS CHEMICAL BIOLOGY, 2012, 7 (11) :1848-1857

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