Assessment of a chemically induced model of lung squamous cell carcinoma in mice by 18F-FDG small-animal PET

被引:13
作者
Ambrosin, Valentina
Nani, Cristina
Pettinato, Cinzia
Fini, Milena
D'Errico, Antonia
Trepidi, Silvia
Spinelli, Antonello
Al-Nahhas, Adil
Rubello, Domenico
Zompatori, Maurizio
Fabbri, Mario
Franchi, Roberto
Fanti, Stefano
机构
[1] Hammersmith Hosp, Dept Nucl Med, London W12 0HS, England
[2] Univ Bologna, Policlin S Orsola Malpighi, Dept Nucl Med, Bologna, Italy
[3] Univ Bologna, Ist A Valsalva, Dept Surg, Bologna, Italy
[4] Univ Bologna, Dept Pulm, Bologna, Italy
[5] Ist Ric Codivilla Putti, Ist Ortoped Rizzoli, Dept Pathol, Bologna, Italy
[6] Osped S Maria Misericordia, Nucl Med Serv, Rovigo, Italy
[7] Univ Parma, Dept Radiol, I-43100 Parma, Italy
关键词
small animal PET; F-18-FDG; squamous cell carcinoma; tumor induction;
D O I
10.1097/MNM.0b013e32823f9ffa
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Background Small-animal imaging has become a relevant research field in pre-clinical oncology. In particular, metabolic information provided by small-animal positron emission tomography (PET) is very useful to closely monitor tumour growth and assess therapy response in murine models of human disease. There are various murine models for human lung adenocarcinoma, but those for squamous cell lung carcinoma, the most common form of human cancer, are lacking. Aim To assess the feasibility of F-18-FDG small-animal PET to monitor tumour growth in a chemically induced model of squamous cell carcinoma of the lung. Materials and methods Nineteen NIH Swiss mice were skin painted by N-nitroso-tris-chloroethylurea (NTCU) twice a week, with a 3 day interval, for 8 months and 10 NIH Swiss mice skin painted with NTCU solvent (acetone) were used as controls. F-18-FDG PET was performed under sevofluorane anaesthesia and oxygen supplementation at 2, 4, 6 and 8 months from initial treatment. Images were assessed by visual analysis and semi-quantitatively. When a diffuse distribution of tumour was noted, the mean of the counts/pixel measured at three lung levels, corrected for the effective dose injected and for decay, was used for comparison between mutagen-painted and control mice. Pathological evaluation was carried out from the time of the first positive PET results in a subgroup of the whole population to assess correlation with PET findings. Small animal CT was performed at 8 months in another subgroup. Results In both terms of visual analysis and measurement of total lung activity, 18F-FDG PET at 2 and 4 months from initial treatment were comparable in mutagen-painted and controls. At 6 months, PET images showed a faint and diffuse uptake over both lung fields in mutagen-painted mice with multiple focal areas of increased tracer uptake that merged into confluent masses at 8 months and seriously subverting lung architecture on computed tomography. Total lung activity was significantly higher in mutagen-painted versus control mice at 6 (P=0.00000668) and 8 months (P=0.00000043) from initial treatment and paralleled the progressive lung involvement and histological severity. Conclusions F-18-DG PET may be useful in the assessment of this chemically induced murine model of lung squamous cells carcinoma. The total lung activity may be used as a measure of tumour metabolic activity of the tumour-bearing animals and may be useful in new drug testing studies. Nucl Med Commun 28:647-652 (c) 2007 Lippincott Williams & Wilkins.
引用
收藏
页码:647 / 652
页数:6
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