Detection of Listeria monocytogenes in Italian-style soft cheeses

被引：30

作者：

Longhi, C ^{[1
]}

Maffeo, A ^{[1
]}

Penta, M ^{[1
]}

Petrone, G ^{[1
]}

Seganti, L ^{[1
]}

Conte, MP ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dept Publ Hlth Sci, I-00185 Rome, Italy

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2003年 / 94卷 / 05期

关键词：

actA gene; DNA detection; Listeria monocytogenes; polymerase chain reaction; soft cheese;

D O I：

10.1046/j.1365-2672.2003.01921.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: A rapid detection system specific for Listeria monocytogenes and based on the polymerase chain reaction (PCR) was developed. Methods and Results: Primers annealing to the coding region of the actA gene, critically involved in virulence and capable of discrimination between two different alleles naturally occurring in L. monocytogenes , have been utilized. The procedure was applied to recover L. monocytogenes cells in artificially contaminated fresh Italian soft cheeses (mozzarella, crescenza and ricotta). Low levels of L. monocytogenes were detected in mozzarella and crescenza homogenates (0.04-0.4 and 4 CFU g(-1) , respectively) whereas in ricotta the detection limit was higher (40 CFU g(-1)). Conclusions: This PCR-based assay is highly specific as primers used recognize the DNA from different L. monocytogenes strains of clinical and food origin, while no amplification products result with any other Listeria spp. strains. Significance and Impact of the Study: This study highlighted a low-cost and rapid procedure that can be appropriated for the detection in real time of low L. monocytogenes levels in soft cheese.

引用

页码：879 / 885

页数：7

共 40 条

[31] Detection of pathogenic and spoilage micro-organisms in food with the polymerase chain reaction [J].

Scheu, PM ;

Berghof, K ;

Stahl, U .

FOOD MICROBIOLOGY, 1998, 15 (01) :13-31

[32] EPIDEMIOLOGY OF HUMAN LISTERIOSIS [J].

SCHUCHAT, A ;

SWAMINATHAN, B ;

BROOME, CV .

CLINICAL MICROBIOLOGY REVIEWS, 1991, 4 (02) :169-183

[33]

Slutsker L., 1999, Listeria, listeriosis and food safety., P75

[34] BEHAVIOR OF LISTERIA-MONOCYTOGENES IN MOZZARELLA CHEESE IN PRESENCE OF LACTOCOCCUS-LACTIS [J].

STECCHINI, ML ;

AQUILI, V ;

SARAIS, I .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1995, 25 (03) :301-310

[35] ACTIN-FILAMENTS AND THE GROWTH, MOVEMENT, AND SPREAD OF THE INTRACELLULAR BACTERIAL PARASITE, LISTERIA-MONOCYTOGENES [J].

TILNEY, LG ;

PORTNOY, DA .

JOURNAL OF CELL BIOLOGY, 1989, 109 (04) :1597-1608

[36] The use of immuno-magnetic separation (IMS) as a tool in a sample preparation method for direct detection of L-monocytogenes in cheese [J].

Uyttendaele, M ;

Van Hoorde, I ;

Debevere, J .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2000, 54 (03) :205-212

[37] Listeria pathogenesis and molecular virulence determinants [J].

Vázquez-Boland, JA ;

Kuhn, M ;

Berche, P ;

Chakraborty, T ;

Domínguez-Bernal, G ;

Goebel, W ;

González-Zorn, B ;

Wehland, J ;

Kreft, J .

CLINICAL MICROBIOLOGY REVIEWS, 2001, 14 (03) :584-+

[38] NUCLEOTIDE-SEQUENCE OF THE LECITHINASE OPERON OF LISTERIA-MONOCYTOGENES AND POSSIBLE ROLE OF LECITHINASE IN CELL-TO-CELL SPREAD [J].

VAZQUEZBOLAND, JA ;

KOCKS, C ;

DRAMSI, S ;

OHAYON, H ;

GEOFFROY, C ;

MENGAUD, J ;

COSSART, P .

INFECTION AND IMMUNITY, 1992, 60 (01) :219-230

[39] Behaviour of Listeria monocytogenes during the traditional manufacture of water-buffalo Mozzarella cheese [J].

Villani, F ;

Pepe, O ;

Mauriello, G ;

Moschetti, G ;

Sannino, L ;

Coppola, S .

LETTERS IN APPLIED MICROBIOLOGY, 1996, 22 (05) :357-360

[40] Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential [J].

Wiedmann, M ;

Bruce, JL ;

Keating, C ;

Johnson, AE ;

McDonough, PL ;

Batt, CA .

INFECTION AND IMMUNITY, 1997, 65 (07) :2707-2716

← 1 2 3 4 →