Molecular typing by pulsed-field gel electrophoresis of Flavobacterium psychrophilum isolates derived from Japanese fish

被引:42
作者
Arai, H.
Morita, Y.
Izumi, S.
Katagiri, T.
Kimura, H.
机构
[1] Natl Inst Infect Dis, Tokyo 2080011, Japan
[2] Gunma Prefectural Fisheries Expt Stn, Maebashi, Gunma, Japan
[3] Tokyo Univ Marine Sci & Technol, Lab Fish Hlth Management, Course Aquat Biosci, Grad Sch Marine Sci & Technol, Tokyo, Japan
[4] Gunma Prefectural Inst Publ Hlth & Environm Sci, Maebashi, Gunma, Japan
关键词
ayu; epidemiology; Flavobacterium psychrophilum; Japan; molecular typing; pulsed-field gel electrophoresis;
D O I
10.1111/j.1365-2761.2007.00809.x
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Sixty-four isolates of Flavobacterium psychrophilum from ayu, Plecoglossus altivelis altivelis (Temminck & Schlegel), and other fish (n = 16) in Japan and the type strain (NCIMB 1947(T)) were typed using pulsed-field gel electrophoresis (PFGE) with endonuclease BlnI and XhoI. These isolates were classified into 20 clusters and 42 genotypes by PFGE analysis. The most predominant cluster of isolates from ayu was cluster XII (n = 20), followed by clusters XVII, XVI, XX, XI, IX, X, XIII and XV; the remaining 17 isolates from other fish were divided into clusters I, II, III, IV, V, VI, VII, VIII, XIV, XVIII and XIX. The PFGE genotype of isolates from ayu clearly differed from those of other fish. The isolates from ayu in Gunma Prefecture belonged to clusters XII, XVI, XVII and XX, and the strains of three of these clusters (XII, XVII and XX) were isolated from ayu in 15 of 19 prefectures. PFGE typing enabled more accurate classification of isolates into clusters than previously achieved by analysing the restriction fragment length polymorphism of PCR products. These results suggest that F. psychrophilum isolated from ayu and other fish are genetically different and strains with several PFGE types have spread within Japan.
引用
收藏
页码:345 / 355
页数:11
相关论文
共 40 条
[1]   An investigation on the distribution of Flavobacterium psychrophilum in the Umikawa River [J].
Amita, K ;
Hoshino, M ;
Honma, T ;
Wakabayashi, H .
FISH PATHOLOGY, 2000, 35 (04) :193-197
[2]   LABORATORY INVESTIGATION OF A MULTISTATE FOOD-BORNE OUTBREAK OF ESCHERICHIA-COLI O157-H7 BY USING PULSED-FIELD GEL-ELECTROPHORESIS AND PHAGE TYPING [J].
BARRETT, TJ ;
LIOR, H ;
GREEN, JH ;
KHAKHRIA, R ;
WELLS, JG ;
BELL, BP ;
GREENE, KD ;
LEWIS, J ;
GRIFFIN, PM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (12) :3013-3017
[3]  
Bäumler AJ, 1998, INFECT IMMUN, V66, P4579
[4]   Random amplified polymorphic DNA analysis provides rapid differentiation among isolates of the fish pathogen Flavobacterium psychrophilum and among Flavobacterium species [J].
Chakroun, C ;
Urdaci, MC ;
Faure, D ;
Grimont, F ;
Bernardet, JF .
DISEASES OF AQUATIC ORGANISMS, 1997, 31 (03) :187-196
[5]   Fingerprinting of Flavobacterium psychrophilum isolates by ribotyping and plasmid profiling [J].
Chakroun, C ;
Grimont, F ;
Urdaci, MC ;
Bernardet, JF .
DISEASES OF AQUATIC ORGANISMS, 1998, 33 (03) :167-177
[6]  
García JA, 2000, FEMS MICROBIOL LETT, V190, P163, DOI 10.1016/S0378-1097(00)00322-0
[7]   Molecular epidemiology of an outbreak due to IRT-2 β-lactamase-producing strains of Klebsiella pneumoniae in a geriatric department [J].
Girlich, D ;
Karim, A ;
Poirel, L ;
Cavin, MH ;
Verny, C ;
Nordmann, P .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 45 (04) :467-473
[8]   Screening method for Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones by PCR-restriction fragment length polymorphism [J].
Hirose, K ;
Tamura, K ;
Watanabe, H .
MICROBIOLOGY AND IMMUNOLOGY, 2003, 47 (02) :161-165
[9]   Effect of competitor abundance on feeding territoriality in a grazing fish, the ayu Plecoglossus altivelis [J].
Iguchi, K ;
Hino, T .
ECOLOGICAL RESEARCH, 1996, 11 (02) :165-173
[10]   Outbreaks of coldwater disease in wild ayu and pale chub [J].
Iida, Y ;
Mizokami, A .
FISH PATHOLOGY, 1996, 31 (03) :157-164