Biopotentiality of Bacillus cereus PB45 for nitrogenous waste detoxification in ex situ model

被引：19

作者：

Barman, Prasenjit ^{[1
]}

Kati, Ahmet ^{[2
]}

Mandal, Amit Kumar ^{[1
]}

Bandyopadhyay, Partha ^{[3
]}

Das Mohapatra, Pradeep Kumar ^{[1
]}

机构：

[1] Vidyasagar Univ, Dept Microbiol, Midnapore 721102, W Bengal, India

[2] Yeditepe Univ, Dept Genet & Bioengn, Fac Engn, TR-34755 Istanbul, Turkey

[3] Biostadt India Ltd, Poonam Chambers A Wing, 6th Floor,Dr AB Rd, Mumbai 400018, Maharashtra, India

来源：

AQUACULTURE INTERNATIONAL | 2017年 / 25卷 / 03期

关键词：

Bacillus cereus PB45; Heterotrophic nitrification; Aerobic denitrification; Water quality management; SIMULTANEOUS HETEROTROPHIC NITRIFICATION; NITRIFYING-DENITRIFYING BACTERIUM; AEROBIC DENITRIFICATION; WATER TREATMENT; REMOVAL; GENES; CULTURE; NITRATE; SHRIMP; CAPABILITY;

D O I：

10.1007/s10499-016-0105-y

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

A bacterial strain PB45 was isolated from an intensive shrimp culture pond, and its nitrification capability was studied. On the basis of 16S ribosomal DNA (rDNA) analysis, strain PB45 was identified as Bacillus cereus PB45. PB45 has the potential to remove NH4+-N,NO3--N, and NO2--N at the rates of 66.6, 79.4, and 50%, respectively. The optimum pH, temperature, and dissolved oxygen for the highest nitrification process of the PB45 were 8.0, 30 degrees C, and 6.68 mg/l (200 rpm), respectively. PB45 could express periplasmic nitrate reductase (nap Lambda), hydroxylamine oxidoreductase (hao), and nitrite reductase (nirS) which are essential to complete heterotrophic nitrification and aerobic denitrification process. Removal of NH4+-N,NO3--N, and NO2--N in an aquarium base shrimp wastewater system was recorded to be 99.76, 99.37, and 99.72%, respectively, by PB45. This result indicated that PB45 has the prospect for future full-scale application in commercial shrimp culture.

引用

页码：1167 / 1183

页数：17

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