Detailed Phenolic Characterization of Protea Pure and Hybrid Cultivars by Liquid Chromatography-Ion Mobility-High Resolution Mass Spectrometry (LC-IM-HR-MS)

被引：21
作者：
Masike, Keabetswe [1 ]
de Villiers, Andre [2 ]
Hoffman, Eleanor W. [3 ]
Brand, D. Jacobus [4 ]
Causon, Tim [5 ]
Stander, Maria A. [1 ,6 ]
机构：
[1] Stellenbosch Univ, Dept Biochem, Private Bag X1, ZA-7602 Stellenbosch, South Africa
[2] Stellenbosch Univ, Dept Chem & Polymer Sci, Private Bag X1, ZA-7602 Stellenbosch, South Africa
[3] Stellenbosch Univ, Dept Hort Sci, Private Bag X1, ZA-7602 Stellenbosch, South Africa
[4] Stellenbosch Univ, Dept Chem, Cent Analyt Facil, NMR Unit, ZA-7602 Stellenbosch, South Africa
[5] Univ Nat Resources & Life Sci BOKU, Inst Analyt Chem, Dept Chem, A-1180 Vienna, Austria
[6] Stellenbosch Univ, Cent Analyt Facil, Private Bag X1, ZA-7602 Stellenbosch, South Africa
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2020年 / 68卷 / 02期
基金：
芬兰科学院; 新加坡国家研究基金会;
关键词：
proteaceae; Protea; UHPLC-MSE; ion mobility (IM) spectrometry; NMR spectroscopy; phenolics; anthocyanins; caffeoyl-O-polygalatol (1,5-anhydro-(6-O-caffeoyl]- sorbitol(glucitol)); DIODE-ARRAY DETECTION; COLLISION CROSS-SECTION; ELECTROSPRAY-IONIZATION; STRUCTURAL-CHARACTERIZATION; POPULATION DIFFERENTIATION; ANALYTICAL STRATEGY; WINE ANTHOCYANINS; IDENTIFICATION; TIME; L;
D O I：
10.1021/acs.jafc.9b06361
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
In this study we report a detailed investigation of the polyphenol composition of Protea pure (P. cynaroides and P. neriifolia) and hybrid cultivars (Black beauty and Limelight). Aqueous methanol extracts of leaf and bract tissues were analyzed by ultrahigh pressure liquid chromatography hyphenated to photodiode array and ion mobility-high resolution mass spectrometric (UHPLC-PDA-IM-HR-MS) detection. A total of 67 metabolites were characterized based on their relative reversed phase (RP) retention, UV-vis spectra, low and high collision energy HR-MS data, and collisional cross section (CCS) values. These metabolites included 41 phenolic acid esters and 25 flavonoid derivatives, including 5 anthocyanins. In addition, an undescribed hydroxycinnamic acid-polygalatol ester, caffeoyl-O-polygalatol (1,5-anhydro-[6-O-caffeoy1]-sorbitol(glucitol)) was isolated and characterized by 1D and 2D NMR for the first time. This compound and its isomer are shown to be potential chemo-taxonomic markers.
引用
收藏
页码：485 / 502
页数：18
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