The microbiome of the invertebrate model host Galleria mellonella is dominated by Enterococcus

被引:49
作者
Allonsius, Camille Nina [1 ]
Van Beeck, Wannes [1 ]
De Boeck, Ilke [1 ]
Wittouck, Stijn [1 ]
Lebeer, Sarah [1 ]
机构
[1] Univ Antwerp, Dept Biosci Engn, Res Grp Environm Ecol & Appl Microbiol, Groenenborgerlaan 171, B-2020 Antwerp, Belgium
关键词
Galleria mellonella; Greater wax moth; Microbiota; Enterococcus; Invertebrate host; Low diversity;
D O I
10.1186/s42523-019-0010-6
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background The popularity of Galleria mellonella as invertebrate model is increasing rapidly, because it forms an attractive alternative to study bacterial, fungal and viral infections, toxin biology, and to screen antimicrobial drugs. For a number of vertebrate and invertebrate animal and plant models, it has been established that the commensals present within the microbial communities on various host surfaces will influence the host's immune and growth development state and the colonization capacity of newly introduced micro-organisms. The microbial communities of Galleria mellonella larvae have, however, not yet been well characterized. Results In this study, we present the bacterial communities that were found by 16S rRNA amplicon sequencing on different body sites of G. mellonella larvae. These communities showed very little diversity and were mostly dominated by one Enterococcus taxon. In addition, we found that the production conditions (as 'bait' for fishing or under more controlled 'research grade' conditions - with or without hormones and antibiotics) appear to have little impact on the microbiota of the larvae. Conclusions Establishment of the simplicity of the microbiota of G. mellonella larvae underlines the potential of the larvae as a model host system for microbiome-host interactions.
引用
收藏
页数:7
相关论文
共 24 条
[1]   Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation [J].
Bates, Jennifer M. ;
Mittge, Erika ;
Kuhlman, Julie ;
Baden, Katrina N. ;
Cheesman, Sarah E. ;
Guillemin, Karen .
DEVELOPMENTAL BIOLOGY, 2006, 297 (02) :374-386
[2]   Frequent Replenishment Sustains the Beneficial Microbiome of Drosophila melanogaster [J].
Blum, Jessamina E. ;
Fischer, Caleb N. ;
Miles, Jessica ;
Handelsman, Jo .
MBIO, 2013, 4 (06)
[3]  
Callahan BJ, 2016, NAT METHODS, V13, P581, DOI [10.1038/NMETH.3869, 10.1038/nmeth.3869]
[4]   Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample [J].
Caporaso, J. Gregory ;
Lauber, Christian L. ;
Walters, William A. ;
Berg-Lyons, Donna ;
Lozupone, Catherine A. ;
Turnbaugh, Peter J. ;
Fierer, Noah ;
Knight, Rob .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 :4516-4522
[5]   Galleria mellonella as a model host for microbiological and toxin research [J].
Champion, Olivia L. ;
Wagley, Sariqa ;
Titball, Richard W. .
VIRULENCE, 2016, 7 (07) :840-845
[6]   ggplot2: Elegant Graphics for Data Analysis [J].
Ginestet, Cedric .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES A-STATISTICS IN SOCIETY, 2011, 174 :245-245
[7]   Oxytetracycline reduces the diversity of tetracycline-resistance genes in the Galleria mellonella gut microbiome [J].
Ignasiak, Katarzyna ;
Maxwell, Anthony .
BMC MICROBIOLOGY, 2018, 18
[8]   Deriving accurate microbiota profiles from human samples with low bacterial content through post-sequencing processing of Illumina MiSeq data [J].
Jervis-Bardy, Jake ;
Leong, Lex E. X. ;
Marri, Shashikanth ;
Smith, Renee J. ;
Choo, Jocelyn M. ;
Smith-Vaughan, Heidi C. ;
Nosworthy, Elizabeth ;
Morris, Peter S. ;
O'Leary, Stephen ;
Rogers, Geraint B. ;
Marsh, Robyn L. .
MICROBIOME, 2015, 3
[9]   Host and Symbiont Jointly Control Gut Microbiota during Complete Metamorphosis [J].
Johnston, Paul R. ;
Rolff, Jens .
PLOS PATHOGENS, 2015, 11 (11)
[10]  
Kavanagh Kevin, 2010, Fungal Biology Reviews, V24, P79, DOI 10.1016/j.fbr.2010.04.001