Multilineage Differentiation Potential of Fibroblast-like Stromal Cells Derived from Human Skin

被引:1
作者
Huang, Hsing-, I [1 ,2 ]
Chen, Shao-Kuan [3 ,4 ]
Ling, Qing-Dong [3 ,5 ]
Chien, Chih-Cheng [4 ,6 ]
Liu, Hsiao-Tung [1 ,2 ]
Chan, Shu-Hui [3 ]
机构
[1] Chang Gung Univ, Dept Med Biotechnol & Lab Sci, 259 Wen Hwa 1st Rd, Kwei Shan Tao Yuan 221, Taiwan
[2] Chang Gung Univ, Res Ctr Emerging Viral Infect, Kwei Shan Tao Yuan 221, Taiwan
[3] Cathay Gen Hosp, Cathay Med Res Inst, Taipei, Taiwan
[4] Cathay Gen Hosp, Hsi Chih, Taiwan
[5] Natl Cent Univ, Grad Inst Syst Biol & Bioinformat, Tao Yuan, Taiwan
[6] Fu Jen Catholic Univ, Dept Med, Taipei, Taiwan
关键词
MESENCHYMAL STEM-CELLS; BONE-MARROW; MULTIPOTENT CELLS; AMNIOTIC-FLUID; POPULATION; EXPRESSION; PROGENITORS; PRECURSORS; DERMIS; BLOOD;
D O I
10.1089/ten.tea.2009.0431
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Adult stem cells that reside in adult tissues have been deemed to possess great potential for clinical application because of their capabilities of self-renewal and differentiation. However, the limitations such as infection risks and low isolation rate make the search for appropriate source to be continued. Here, we demonstrate isolation of progenitors from human foreskin tissue samples, which have fibroblast-like morphology and could be easily propagated for more than 50 passages. These foreskin-derived fibroblast-like stromal cells (FDSCs) expressed CD90, CD105, CD166, CD73, SH3, and SH4, which is similar to the immunophenotypes of human bone marrow-derived mesenchymal stem cells. In comparison with Hs68, the human fibroblast cell line, FDSCs are positive for CD105 and absent for CD54 expression. Further, FDSCs could be induced to differentiate into osteocytes, adipocytes, neural cells, smooth muscle cells, Schwann-like cells, and hepatocyte-like cells. Interestingly, when cultured in Dulbecco's modified Eagle's medium/F12 medium, FDSCs can form spheres with increased expression levels of fibronectin and vimentin. In conclusion, foreskin can serve as a valuable source of multipotent cells with the capabilities for endodermal, mesodermal, and ectodermal cells. Coupled with the advantages of their easy access, isolation, and propagation, these foreskin-derived stromal cells might be of potential use in future studies in stem cell differentiation and therapeutic application.
引用
收藏
页码:1491 / 1501
页数:11
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