Silencing of Ribosomal Protein L34 (RPL34) Inhibits the Proliferation and Invasion of Esophageal Cancer Cells

被引:33
|
作者
Fan, Huijie [1 ]
Li, Jing [1 ]
Jia, Yongxu [1 ]
Wu, Jingjing [1 ]
Yuan, Long [2 ]
Li, Mingjun [1 ]
Wei, Jiangqi [3 ]
Xu, Benling [4 ]
机构
[1] Zhengzhou Univ, Affiliated Hosp 1, Dept Oncol, 1 Jianshe East Rd, Zhengzhou 450052, Henan, Peoples R China
[2] Zhengzhou Univ, Affiliated Canc Hosp, Dept Surg, Zhengzhou, Henan, Peoples R China
[3] First Renmin Hosp Xinxiang City, Dept Surg, Xinxiang, Peoples R China
[4] Zhengzhou Univ, Affiliated Canc Hosp, Dept Canc Biotherapy, Zhengzhou, Henan, Peoples R China
关键词
Ribosomal protein L34 (RPL34); Esophageal cancer; Proliferation; Invasion; PI3K/Akt pathway; PI3K/AKT SIGNALING PATHWAY; TUMOR-GROWTH; EPIDEMIOLOGY; METASTASIS; PATHOGENESIS; EXPRESSION; CARCINOMA;
D O I
10.3727/096504016X14830466773541
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Ribosomal protein L34 (RPL34) belongs to the L34E family of ribosomal proteins and contains a zinc finger motif. Aberrant expression of RPL34 has been reported in several human malignancies. However, the precise role and potential underlying mechanisms of RPL34 in human esophageal cancer remain largely unknown. Thus, the objective of this study was to investigate the role of RPL34 in esophageal cancer progression. Our results showed that the expression of RPL34 at both the mRNA and protein levels was frequently upregulated in esophageal cancer cell lines. Knockdown of RPL34 efficiently inhibited esophageal cancer cell proliferation, migration, and invasion in vitro. Mechanistically, knockdown of RPL34 significantly downregulated the protein expression level of p-PI3K and p-Akt in esophageal cancer cells. Finally, knockdown of RPL34 attenuated tumor growth in nude mice. In conclusion, our study revealed that RPL34 functions as an oncogene that modulates the proliferation and metastasis of esophageal cancer cells, in part, by the inactivation of the PI3K/Akt signaling pathway. Thus, these findings suggest that RPL34 may serve as a potential therapeutic target for the treatment of esophageal cancer.
引用
收藏
页码:1061 / 1068
页数:8
相关论文
共 50 条
  • [1] Ribosomal protein L34 promotes the proliferation, invasion and metastasis of pancreatic cancer cells
    Wei, Feng
    Ding, Lijuan
    Wei, Zhentong
    Zhang, Yandong
    Li, Yang
    Luo Qinghua
    Ma, Yuteng
    Guo, Liang
    Lv, Guoyue
    Liu, Yan
    ONCOTARGET, 2016, 7 (51): : 85259 - 85272
  • [2] Suppression of RPL34 Inhibits Tumor Cell Proliferation and Promotes Apoptosis in Glioblastoma
    Du, Changwang
    Wang, Taoning
    Jia, Jinning
    Li, Junjun
    Xiao, Yi
    Wang, Jia
    Mao, Ping
    Wang, Ning
    Shi, Luoning
    Wang, Maode
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2022, 194 (08) : 3494 - 3506
  • [3] Suppression of RPL34 Inhibits Tumor Cell Proliferation and Promotes Apoptosis in Glioblastoma
    Changwang Du
    Taoning Wang
    Jinning Jia
    Junjun Li
    Yi Xiao
    Jia Wang
    Ping Mao
    Ning Wang
    Luoning Shi
    Maode Wang
    Applied Biochemistry and Biotechnology, 2022, 194 : 3494 - 3506
  • [4] 羊驼皮肤中核糖体蛋白L34(RPL34)基因表达的分析
    张俊珍
    范瑞文
    李鹏飞
    白瑞
    朱芷葳
    董常生
    激光生物学报, 2010, (04) : 479 - 482+487
  • [5] Sequencing and characterization of the Xenopus laevis ribosomal protein L34 cDNA
    Vaccaro, MC
    GENE, 2003, 318 : 163 - 167
  • [6] RPL34-AS1-induced RPL34 inhibits cervical cancer cell tumorigenesis via the MDM2-P53 pathway
    Zhu, Yuanhang
    Ren, Chenchen
    Jiang, Dongyuan
    Yang, Li
    Chen, Yannan
    Li, Feiyan
    Wang, Baojin
    Zhang, Yali
    CANCER SCIENCE, 2021, 112 (05) : 1811 - 1821
  • [7] Regulatory roles of benzyl adenine and sucrose during wound response of the ribosomal protein gene, rpL34
    Yu, GH
    An, G
    PLANT CELL AND ENVIRONMENT, 2000, 23 (12): : 1363 - 1371
  • [8] Knockdown of RPL34 inhibits the proliferation and migration of glioma cells through the inactivation of JAK/STAT3 signaling pathway
    Ji, Peigang
    Wang, Liang
    Liu, Jinghui
    Mao, Ping
    Li, Ruichun
    Jiang, Haitao
    Lou, Miao
    Xu, Meng
    Yu, Xiao
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2019, 120 (03) : 3259 - 3267
  • [9] THE PRIMARY STRUCTURE OF A GENE ENCODING YEAST RIBOSOMAL-PROTEIN L34
    SCHAAP, PJ
    MOLENAAR, CMT
    MAGER, WH
    PLANTA, RJ
    CURRENT GENETICS, 1984, 9 (01) : 47 - 52
  • [10] Dystroglycan and Mitochondrial Ribosomal Protein L34 Regulate Differentiation in the Drosophila Eye
    Zhan, Yougen
    Melian, Nadia Y.
    Pantoja, Mario
    Haines, Nicola
    Ruohola-Baker, Hannele
    Bourque, Charles W.
    Rao, Yong
    Carbonetto, Salvatore
    PLOS ONE, 2010, 5 (05):