Highly sensitive quantification of site-specific 5-hydroxymethylcytosine at single-base resolution by HpaII-mediated ligation PCR

被引：11
|
作者：
Zhang, Zhenhao [1 ]
Shan, Xiao [1 ]
Zhang, Pengbo [2 ]
Liu, Weiliang [1 ]
Yan, Jingli [1 ]
Li, Zhengping [1 ]
机构：
[1] Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Key Lab Appl Surface & Colloid Chem,Minist Educ, Xian 710062, Shaanxi, Peoples R China
[2] Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Beijing 100083, Peoples R China
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2019年 / 17卷 / 46期
基金：
中国国家自然科学基金;
关键词：
TET PROTEINS; DNA; 5-METHYLCYTOSINE; CONVERSION;
D O I：
10.1039/c9ob02429h
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A highly sensitive HpaII-mediated ligation PCR assay that can precisely discriminate 5hmC from 5mC and C is developed for the quantitative determination of site-specific 5hmC in genomic DNA samples.
引用
收藏
页码：9849 / 9853
页数：5
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