Activities of oxidative enzymes related with oxygen tolerance in Bifidobacterium sp.

被引:0
作者
Shin, SY [1 ]
Park, JH [1 ]
机构
[1] KOREA FOOD RES INST,BUNDANG GU,SONGNAM 463420,SOUTH KOREA
关键词
bifidobacteria; oxygen tolerance; superoxide dismutase; NADH oxidase; NADH peroxidase;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To study the relationship between oxygen tolerance and enzyme activity in the oxygen metabolism of bifidobacteria, the activities of catalase, superoxide dismutase (SOD), NADH oxidase and NADH peroxidase from six typical bifidobacteria and other bacteria were assayed by spectrophotometry. Catalase activity was hardly detected in any of the bifidobacteria tested. SOD activity was detected in every species including the Clostridium species. Tn particular SOD activity was notably high in the aerosensitive Bifidobacterium adolescentis. This fact indicates that SOD activity is not a critical factor to ensure aerotolerance. Aerosensitive B. adolescentis showed very low NADH oxidative enzyme activity whereas other aerotolerant bifidobacteria exhibited considerable activity for the enzymes, It seems that detoxification of H2O2 by NADH oxidative enzymes might be an important factor in improving for aerotolerant bifidobacteria survival rates in an oxygen environment.
引用
收藏
页码:356 / 359
页数:4
相关论文
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