FBPase is in the nuclei of cardiomyocytes

被引：37

作者：

Gizak, A ^{[1
]}

Dzugaj, A ^{[1
]}

机构：

[1] Univ Wroclaw, Inst Zool, Dept Anim Physiol, PL-50205 Wroclaw, Poland

来源：

FEBS LETTERS | 2003年 / 539卷 / 1-3期

关键词：

fructose-1,6-bisphosphatase; cardiomyocyte; nucleus;

D O I：

10.1016/S0014-5793(03)00183-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intracellular localization of FBPase in the cardiac muscle of the pig was studied by immunohistochemistry. In contrast to data from skeletal muscle [Gizak, A., Rakus, D. and Dzugaj, A. (2003) Histol. Histopathol. 18, 135-142], in cardiomyocytes FBPase was present not only in the cytoplasm, but surprisingly, also in the nucleus. Results of the microscopic investigation were confirmed by immunoblotting, measurement of FBPase activity in isolated cardiomyocyte nuclei and by determination of the nuclear FBPase I-0.5 toward adenosine monophosphate (AMP), which was the same as for the purified enzyme. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：51 / 55

页数：5

共 32 条

[11] Leatherbarrow R.J., 1992, GRAFIT 3 0
[12] MIWA I, 1990, BIOCHEM INT, V22, P759
[13] NEWSHOLME EA, 1976, REGULATION METABOLIS, P88
[14] PILKIS SJ, 1981, J BIOL CHEM, V256, P3619
[15] Modulation of DNA polymerases α, δ and ε by lactate dehydrogenase and 3-phosphoglycerate kinase
Popanda, O
Fox, G
Thielmann, HW
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1998, 1397 (01): : 102 - 117
[16] Muscle aldolase decreases muscle FBPase sensitivity toward AMP inhibition
Rakus, D
Dzugaj, A
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 275 (02) : 611 - 616
[17] RAKUS D, 2003, IN PRESS ACTA BIOCH, V50
[18] GLYCOLYTIC-ENZYMES AS DNA-BINDING PROTEINS
RONAI, Z
[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1993, 25 (07): : 1073 - 1076
[19] DISTINGUISHABLE SUBSTRATE POOLS FOR MUSCLE GLYCONEOGENESIS IN LACTATE-SUPPLEMENTED RECOVERY FROM EXERCISE
RYAN, C
RADZIUK, J
[J]. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1995, 269 (03): : E538 - E550
[20] Saez DE, 1996, J CELL BIOCHEM, V63, P453

← 1 2 3 4 →