共 58 条
Electrochemical aptasensor for 17β-estradiol using disposable laser scribed graphene electrodes
被引:58
作者:
Chang, Zhu
[1
]
Zhu, Bicheng
[2
,3
]
Liu, JinJin
[1
]
Zhu, Xu
[1
]
Xu, Maotian
[1
]
Travas-Sejdic, Jadranka
[2
,3
]
机构:
[1] Shangqiu Normal Univ, Coll Chem & Chem Engn, Henan Key Lab Biomol Recognit & Sensing, Henan Joint Int Res Lab Chemo Biosensing & Early, Shangqiu 476000, Henan, Peoples R China
[2] Univ Auckland, Sch Chem Sci, Polymer Biointerface Ctr, Private Bag 92019, Auckland 1142, New Zealand
[3] Victoria Univ Wellington, MacDiarmid Inst Adv Mat & Nanotechnol, POB 600, Wellington, New Zealand
关键词:
E2;
aptasensor;
Split aptamer fragments;
Differential pulse voltammetry;
Host-guest interaction;
Laser scribed graphene electrode;
SENSITIVE DETECTION;
GOLD NANOPARTICLES;
STRANDED-DNA;
APTAMER;
BIOSENSOR;
CARBON;
ACID;
ELECTROPOLYMERIZATION;
MOLECULES;
FERROCENE;
D O I:
10.1016/j.bios.2021.113247
中图分类号:
Q6 [生物物理学];
学科分类号:
071011 ;
摘要:
1713-Estradiol (E2), the strongest of the three major physiological estrogens in females, is an important factor in the female reproductive system. The abnormal level of E2 causes health issues, such as weak bones, urinary tract infections and even depression. Here, we present a novel, sensitive and selective, electrochemical aptasensor for detection of 1713-estradiol (E2). The E2 recognition aptamer was split into two fragments: the first fragment, functionalised with adamantane, is attached to poly(13-cyclodextrin) (poly(13-CD))-modified electrode surface through host-guest interactions between the adamantane and poly(13-CD). The second fragment, labelled with gold nanoparticles, forms the stem-loop structure with the first fragment only in the presence of E2. That specific recognition process triggers the change in the electrochemical signal (a change in the peak current from reduction of AuNPs), recorded by means of differential pulse voltammetry (DPV). The feasibility of the sensing design was firstly investigated on the commercially available glass carbon electrodes (GCE), with achieved a linear detection range of 1.0 x 10-13 to 1.0 x 10-8 M and a limit of detection (LoD) 0.7 fM. The sensing methodology was then translated onto single-use, disposable, laser-scribed graphene electrodes (LSGE) on a plastic substrate. The dynamic sensing range of E2 on LSGE was found to be 1.0 x 10-13 to 1.0 x 10-9 M, with a LoD of 63.1 fM, comparable to these of GCE. The successful translation of the developed E2 aptasensor from GCE to low-cost, disposable LSGE highlights a potential of this sensing platform in commercial, portable sensing detection systems for E2 and similar targets of biological interest.
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